{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:08:05Z","timestamp":1791320885215,"version":"4.3.1"},"reference-count":18,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2005,8,30]],"date-time":"2005-08-30T00:00:00Z","timestamp":1125360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,8,30]],"date-time":"2005-08-30T00:00:00Z","timestamp":1125360000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Exp Nephrol"],"abstract":"<i>Background:<\/i> C-reactive protein (CRP) is known to have a direct proinflammatory effect in endothelial cells. However, little is known about the effect of CRP in intrinsic renal cells. We investigated the effects of CRP on the nuclear factor-\u0138B (NF-\u0138B) activation and monocyte chemoattractant protein-1 (MCP-1) gene expression in human mesangial cells and also examined whether intracellular calcium and reactive oxygen species (ROS) were involved in the CRP- induced NF-\u0138B activation. <i>Methods:<\/i> NF-\u0138B binding activity and MCP-1 mRNA expression were measured by electrophoretic mobility shift assay and Northern blot analysis, respectively.Intracellular calcium was monitored by confocal microscopy using calcium sensitive dye, Fluo-3 and intracellular ROS production was determined, using 2\u2032,7\u2032-dichlorofluorescin diacetate. <i>Results:<\/i> CRP increased NF-\u0138B binding activity in a dose-dependent manner (12.5\u2013100 \u00b5g\/ml), which was induced within 1 h after incubation and peaked around 3 h. CRP also increased the MCP-1 mRNA expression via activation of NF-\u0138B. Both intracellular calcium and ROS was induced by CRP. Calcium chelator, BAPTA-AM and anti-oxidants such as N-acetylcysteine and tiron suppressed CRP-induced NF-\u0138B activation. <i>Conclusion:<\/i> CRP exerted a proinflammatory effect in human mesangial cells by inducing MCP-1 gene expression via NF-\u0138B activation, which was mediated, at least in part, through intracellular calcium and ROS.<\/jats:p>","DOI":"10.1159\/000087940","type":"journal-article","created":{"date-parts":[[2005,8,30]],"date-time":"2005-08-30T12:24:58Z","timestamp":1125404698000},"page":"e165-e172","source":"Crossref","is-referenced-by-count":23,"title":["C-Reactive Protein Induces NF-\u03baB Activation through Intracellular Calcium and ROS in Human Mesangial Cells"],"prefix":"10.1159","volume":"101","author":[{"given":"Jai Won","family":"Chang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choung Soo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon Bae","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su Kil","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Sik","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Koo","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2005,8,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM199902113400607"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1161%2F01.CIR.0000015126.83143.B4"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002%2Fimmu.200390018"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002%2Fart.10930"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.271.30.17779"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1093%2Fndt%2F16.9.1764"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1159%2F000045641"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1291%2Fhypres.23.521"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159%2F000065441"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1161%2F01.CIR.0000061916.95736.E5"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2FS0022-1759%2899%2900100-3"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1161%2F01.CIR.0000033116.22237.F9"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS0008-6363%2802%2900855-6"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS0006-291X%2802%2903009-7"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM200003233421202"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1159%2F000065018"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS0272-6386%2800%2970296-4"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2002.00677.x"}],"container-title":["Nephron Experimental Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/87940","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/87940","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:26:52Z","timestamp":1745465212000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000087940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,8,30]]},"references-count":18,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2005,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000087940","archive":["Portico"],"relation":{},"ISSN":["1660-2129"],"issn-type":[{"value":"1660-2129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,8,30]]}}}