{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:46:25Z","timestamp":1761630385460,"version":"3.40.4"},"reference-count":13,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[2006]]},"abstract":"<i>Background:<\/i> This study aimed to evaluate the diagnostic utilities of monocyte HLA-DR as an infection marker in the identification of early-onset clinical infection and pneumonia in newborn infants. <i>Methods:<\/i> Term newborns in whom infection was suspected when they were <72 h of age were eligible for enrollment in the study. C-reactive protein (CRP), monocyte HLA-DR and neutrophil CD64 expressions were quantitatively measured at the time of sepsis evaluation (0 h) and 24 h afterwards by flow cytometry and standard laboratory method. <i>Results:<\/i> A total of 288 infants with suspected sepsis were investigated, and 93 were found to be clinically infected. There were no significant differences in monocyte HLA-DR expression between the infected, non-infected and control groups at 0 h (median (interquartile range): 13,986 (10,994\u201318,544), 14,234 (12,045\u201317,474) and 18,441 (14,250\u201321,537) antibody phycoerythrin (PE) molecules bound\/cell), and between infected and non-infected infants at 24 h (median (interquartile range): 17,772 (12,933\u201325,167) and 19,406 (14,885\u201324,225) antibody PE molecules bound\/cell). The areas under the receiver operating characteristics (ROC) curves for HLA-DR, CD64 and CRP were 0.52\u20130.54, 0.88\u20130.94 and 0.75\u20130.77, respectively. We were unable to determine an optimal cutoff value for HLA-DR, as the diagnostic utilities of any cutoff point on the ROC curves were unable to satisfy the criteria (i.e. sensitivity and specificity \u226780%) for consideration as an useful diagnostic marker of infection. <i>Conclusions:<\/i> Our findings did not support the use of monocyte HLA-DR alone or in combination with other infection markers in the diagnosis of early-onset clinical infection and pneumonia in term newborns.<\/jats:p>","DOI":"10.1159\/000088288","type":"journal-article","created":{"date-parts":[[2005,9,12]],"date-time":"2005-09-12T12:54:13Z","timestamp":1126529653000},"page":"75-81","source":"Crossref","is-referenced-by-count":32,"title":["Quantitative Measurement of Monocyte HLA-DR Expression in the Identification of Early-Onset Neonatal Infection"],"prefix":"10.1159","volume":"89","author":[{"given":"Pak Cheung","family":"Ng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geng","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kit Man","family":"Chui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Winnie Chiu Wing","family":"Chu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond Pui On","family":"Wong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai Fai","family":"Fok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2006,2,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1542%2Fpeds.108.1.e12"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1136%2Fadc.2002.023838"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2FS0022-3476%2898%2970018-6"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1203%2F01.PDR.0000142586.47798.5E"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1097%2F00007890-199706270-00026"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002%2Fbjs.1800800224"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1097%2F00000658-199902000-00013"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097%2F00003246-199512000-00006"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1542%2Fpir.9-7-219"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1203%2F00006450-200203000-00006"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2249.2001.01471.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159%2F000081980"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-3083.2002.01092.x"}],"container-title":["Neonatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/88288","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/88288","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:29:53Z","timestamp":1745465393000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000088288"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006]]},"references-count":13,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2006,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000088288","archive":["Portico"],"relation":{},"ISSN":["1661-7800","1661-7819"],"issn-type":[{"type":"print","value":"1661-7800"},{"type":"electronic","value":"1661-7819"}],"subject":[],"published":{"date-parts":[[2006]]}}}