{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:23:34Z","timestamp":1773300214254,"version":"3.50.1"},"reference-count":23,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2006,1,9]],"date-time":"2006-01-09T00:00:00Z","timestamp":1136764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,1,9]],"date-time":"2006-01-09T00:00:00Z","timestamp":1136764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Physiol"],"abstract":"<jats:p>&lt;i&gt;Background\/Aims:&lt;\/i&gt; Inducible nitric oxide (NO) synthase (iNOS) generated NO increases in the early phase of Thy-1 glomerulonephritis concurrently with mesangiolysis and reduction in glomerular filtration rate (GFR). Activation of ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine biosynthesis, is upregulated to allow mesangial cell proliferation which constitutes the repair phase in this model. Antiproliferative high-output NO generation inhibits proproliferative ODC activity, thereby temporally separating the early \u2018bactericidal\u2019 phase from the later \u2018growth\u2019 repair phase. &lt;i&gt;Methods:&lt;\/i&gt; Renal function, ODC protein expression, arginine, ornithine, and polyamines by high-performance liquid chromatography, and histological changes were assessed in rats after induction of Thy-1 nephritis with and without NOS inhibition. &lt;i&gt;Results:&lt;\/i&gt; Thy-1 significantly reduced the GFR relative to untreated controls. Treatment with a nonspecific NOS inhibitor, but not a selective iNOS inhibitor, further decreased the GFR at day 1. This implys a protective role for constitutive NOS in the early phase of this inflammatory model. Selective iNOS inhibition abrogated increased plasma NO&lt;sub&gt;2&lt;\/sub&gt;\/NO&lt;sub&gt;3&lt;\/sub&gt; levels in Thy-1 glomerulonephritis, but did not significantly reduce mesangiolysis. However, inhibition of iNOS did result in significantly more nuclei\/glomerulus during the proliferative phase, increasing the hypercellularity component of this disease model. This correlates with increased levels of polyamines, ornithine, and arginine beyond those observed with Thy-1 administration alone. &lt;i&gt;Conclusions:&lt;\/i&gt; These studies provide evidence that NO generation from different NOS isoforms can be protective in the temporal course of Thy-1 glomerulonephritis. The finding that iNOS attenuates hypercellularity in the repair phase of this inflammatory model adds cautionary insight in the therapeutic use of selective iNOS inhibition in vivo.<\/jats:p>","DOI":"10.1159\/000088970","type":"journal-article","created":{"date-parts":[[2005,10,17]],"date-time":"2005-10-17T14:01:44Z","timestamp":1129557704000},"page":"p17-p26","source":"Crossref","is-referenced-by-count":4,"title":["Inhibition of Inducible Nitric Oxide Synthase Alters Thy-1 Glomeruonephritis in Rats"],"prefix":"10.1159","volume":"102","author":[{"given":"Joseph","family":"Satriano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark J.","family":"Lortie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunji","family":"Ishizuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 M.","family":"Valdivielso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beth","family":"Friedman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen A.","family":"Munger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2006,1,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.179.2.651"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.3109%2F07853899509002588"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1996.158"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1991.264"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI119551"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159%2F000020581"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2F0003-2697%2882%2990118-X"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1080%2F003655902762467594"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.1999.00697.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI115849"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.1998.00780.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2249.1997.d01-906.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI118681"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.172.6.1843"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.271.21.12234"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2FS0891-5849%2898%2900092-6"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1990.196"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI115347"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI116118"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1523-1755.1998.00892.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1992.394"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00170166"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00726-004-0078-4"}],"container-title":["Nephron Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/88970","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/88970","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:34:54Z","timestamp":1745465694000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000088970"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,9]]},"references-count":23,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2006,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000088970","archive":["Portico"],"relation":{},"ISSN":["1660-2137"],"issn-type":[{"value":"1660-2137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,1,9]]}}}