{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T08:35:44Z","timestamp":1778661344534,"version":"3.51.4"},"reference-count":26,"publisher":"S. Karger AG","issue":"3-4","license":[{"start":{"date-parts":[[2005,11,11]],"date-time":"2005-11-11T00:00:00Z","timestamp":1131667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,11,11]],"date-time":"2005-11-11T00:00:00Z","timestamp":1131667200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Exp Nephrol"],"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Renal tubular epithelial cells (TECs) function as antigen-presenting cells (APCs) as they constitutively express MHC-II molecules and have the capacity to present peptide antigen to T cells. Nevertheless, co-stimulatory signals provided by TECs for regulating T cell activation have not been fully characterized. We therefore investigated the expression of B7-H1, a member of the B7 superfamily, on TECs under normal or pathologic conditions in vivo and analyzed the regulation and functional role of it after proinflammatory factors treatment in vitro. &lt;i&gt;Methods:&lt;\/i&gt; Immunohistological staining for B7-H1 on cryostat sections of core needle biopsies from patients with different renal diseases was examined. Furthermore, we also detected B7-H1 protein expression on cultured human TECs stimulated by various inflammatory factors and performed TEC\/T-cell co-cultured experiment to determine TEC-associated B7-H1 in regulating CD4&lt;sup&gt;+&lt;\/sup&gt; T cell activation as well as antigen presentation. &lt;i&gt;Results:&lt;\/i&gt; Significant B7-H1 protein was detected in TECs of diseased renal samples. Although the presence of B7-H1 does not have any correlation with clinicopathological variables, marked B7-H1 expression on sections without interstitial inflammation revealed that B7-H1 has some protective function. In vitro, the expression of B7-H1 on TECs was increased after TECs were stimulated with IL-1\u03b1, LPS, TNF-\u03b1, IFN-\u03b3 or anti-CD40. Co-cultured experiments revealed that TEC-related B7-H1 was identified as a strong inhibitor of CD4&lt;sup&gt;+&lt;\/sup&gt; T-cell activation as assessed by increased cytokine production (interleukin-2 and interferon-\u03b3) and expression levels of the T cell activation marker (CD69) in the presence of a neutralizing antibody against B7-H1 (clone MIH1). Interestingly, IL-2 production by C10 T cells after antigen presentation by murine TECs was also enhanced when the B7-H1\/PD-1 pathway was interrupted. &lt;i&gt;Conclusion:&lt;\/i&gt; This study clearly shows that B7-H1 is an inducible renal tubular epithelial antigen that inhibits T cell activation. It is speculated that B7-H1\/PD-1 pathway might play a role in protecting tubular epithelium from immune-mediated damage and active delivery of the B7-H1 inhibitory signal represents a novel therapeutic strategy in autoimmune renal diseases.<\/jats:p>","DOI":"10.1159\/000089686","type":"journal-article","created":{"date-parts":[[2005,11,11]],"date-time":"2005-11-11T07:43:11Z","timestamp":1131694991000},"page":"e81-e92","source":"Crossref","is-referenced-by-count":35,"title":["Expression of B7-H1 in Inflammatory Renal Tubular Epithelial Cells"],"prefix":"10.1159","volume":"102","author":[{"given":"Yongwen","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingbo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyun","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuzhang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2005,11,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI200112080"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.immunol.20.091101.091806"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.2113314"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038%2F35105024"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038%2Fnri1349"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1993.333"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038%2F70932"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.192.7.1027"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.291.5502.319"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.0307252101"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI200419210"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1994.6"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1990.46"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1159%2F000049384"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1097%2F01.ASN.0000017901.77985F"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1002%2Feji.200324270"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1038%2F85330"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1038%2F85339"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016%2FS1074-7613%2803%2900152-3"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1097%2F00007890-198808000-00022"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1991.39"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2249.1993.tb05961.x"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2249.1992.tb05841.x"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00878382"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1997.2"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1997.475"}],"container-title":["Nephron Experimental Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/89686","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/89686","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:43:17Z","timestamp":1745466197000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000089686"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11,11]]},"references-count":26,"journal-issue":{"issue":"3-4","published-online":{"date-parts":[[2006,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000089686","archive":["Portico"],"relation":{},"ISSN":["1660-2129"],"issn-type":[{"value":"1660-2129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,11,11]]}}}