{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T21:18:08Z","timestamp":1775078288518,"version":"3.50.1"},"reference-count":23,"publisher":"S. Karger AG","issue":"4-5","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathophysiol Haemos Thromb"],"published-print":{"date-parts":[[2005]]},"abstract":"<jats:p>Bothrojaracin (BJC) is a selective and potent thrombin inhibitor (&lt;i&gt;K&lt;\/i&gt;&lt;sub&gt;D&lt;\/sub&gt; = 0.6 n&lt;i&gt;M&lt;\/i&gt;) which also binds to prothrombin on proexosite I (&lt;i&gt;K&lt;\/i&gt;&lt;sub&gt;D&lt;\/sub&gt; = 175 n&lt;i&gt;M&lt;\/i&gt;). Incubation of BJC with human or rat plasma produced a band that co-migrates with purified prothrombin-BJC complex. We further analyzed the in vivo anti-thrombotic effect of BJC on a venous thrombosis model in rats that combines stasis and hypercoagulability. The administration of 1 mg\/kg (i.v.) doses of BJC decreased thrombus weight by \u223c95%. Evaluation of the in vivo effect of BJC in mice using a pulmonary thromboembolism model induced by thrombin showed that BJC protects 100% of mice from death. Altogether, our data show that BJC is a potent anti-thrombotic agent that could further help the development of new prothrombin-directed drugs.<\/jats:p>","DOI":"10.1159\/000092416","type":"journal-article","created":{"date-parts":[[2006,5,26]],"date-time":"2006-05-26T13:49:21Z","timestamp":1148651361000},"page":"160-163","source":"Crossref","is-referenced-by-count":18,"title":["Bothrojaracin, a &lt;i&gt;Bothrops jararaca&lt;\/i&gt; Snake Venom-Derived (Pro)Thrombin Inhibitor, as an Anti-Thrombotic Molecule"],"prefix":"10.1159","volume":"34","author":[{"given":"Russolina B.","family":"Zingali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos S.","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariane","family":"Assafim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1via S.","family":"Frattani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robson Q.","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2006,5,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0041-0101%2898%2900126-3"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2FS0300-9084%2800%2901178-0"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1021%2Fbi00091a034"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1432-1033.1997.00550.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.90.3.928"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1021%2Fbi00078a012"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.271.17.10210"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0049-3848%2800%2900260-7"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-5793%2802%2903507-X"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2FS0041-0101%2896%2900176-6"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2FS0305-0491%2898%2910070-6"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0041-0101%2898%2900111-1"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS0041-0101%2899%2900087-2"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS0041-0101%2802%2900358-6"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1021%2Fbi960043l"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.1999.1297"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1159%2F000048073"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1006%2Fabbi.2000.2006"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M001255200"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1110%2Fps.09001"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1378%2Fchest.119.1_suppl.64S"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.thromres.2003.10.014"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI575"}],"container-title":["Pathophysiology of Haemostasis and Thrombosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/92416","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/92416","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:03:33Z","timestamp":1745463813000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000092416"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005]]},"references-count":23,"journal-issue":{"issue":"4-5","published-online":{"date-parts":[[2006,5,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000092416","archive":["Portico"],"relation":{},"ISSN":["1424-8832","1424-8840"],"issn-type":[{"value":"1424-8832","type":"print"},{"value":"1424-8840","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005]]}}}