{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:13:11Z","timestamp":1791288791944,"version":"4.3.0"},"reference-count":18,"publisher":"S. Karger AG","issue":"4-5","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathophysiol Haemos Thromb"],"published-print":{"date-parts":[[2005]]},"abstract":"Snake venoms contain four classes of metalloproteases that all have a typical zinc-chelating sequence (HEXXHGXXH). N-terminal sequences and internal sequences of different purified metalloproteases were determined using Edman sequencing and LC MS\/MS technique. Oligonucleotideswere designed and used as primers for cDNA cloning from <i>Vipera lebetina<\/i> venom gland cDNA library. We found that isoforms of fibrinolytic enzyme lebetase Le-4 and Le-3 are synthesized in different way: Le-4 is synthesized as P-I type metalloprotease, Le-3 is synthesized with disintegrin-like domain as P-II type protease and processed post-translationally. An endothelial cell apoptosis-inducing heterodimeric glycosylated metalloprotease, <i>V. lebetina<\/i> apoptosis-inducing protease (VLAIP), belongs to P-III type containing metalloprotease, disintegrin-like and cysteine-rich domains. All these enzymes hydrolyze the A\u03b1-chain and more slowly the B\u03b2-chain of fibrinogen. Treatment of HUVEC cells with VLAIP induces changes in the attachment of cells to the substrate and causes apoptosis.<i> V. lebetina <\/i>venom contains also P-IV type-specific coagulant factor X activator (VLFXA) that cleaves the Arg<sup>52<\/sup>-Ile<sup>53<\/sup> bond in the heavy chain of human factor X. VLFXA is a glycoprotein composed of a heavy chain and two C-type lectin-like light chains linked by disulfide bonds. The heavy and light chains of VLFXA are synthesized from different genes.<\/jats:p>","DOI":"10.1159\/000092426","type":"journal-article","created":{"date-parts":[[2006,5,26]],"date-time":"2006-05-26T13:49:21Z","timestamp":1148651361000},"page":"209-214","source":"Crossref","is-referenced-by-count":14,"title":["<i>Vipera lebetina<\/i> Venom Contains All Types of Snake Venom Metalloproteases"],"prefix":"10.1159","volume":"34","author":[{"given":"J.","family":"Siigur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"T\u00f5nism\u00e4gi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Trummal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Aasp\u00f5llu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Samel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Vija","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Subbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Kalkkinen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Siigur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2006,5,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2F0041-0101%2892%2990040-C"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.toxicon.2005.02.026"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.toxicon.2005.02.027"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2F0163-7258%2894%2990049-3"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1006%2Fabbi.1994.1026"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2F0076-6879%2895%2948023-4"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.toxicon.2005.02.012"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0955-0674%2898%2980042-2"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0049-3848%2898%2900009-7"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.1996.1012"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1042%2FBJ20021739"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002%2Fpro.5560010505"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.toxicon.2005.03.008"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS0041-0101%2802%2900179-4"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1042%2FBJ20020449"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.1999.1322"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1432-1327.2001.02246.x"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.1996.0414"}],"container-title":["Pathophysiology of Haemostasis and Thrombosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/92426","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/92426","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:03:40Z","timestamp":1745463820000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000092426"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005]]},"references-count":18,"journal-issue":{"issue":"4-5","published-online":{"date-parts":[[2006,5,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000092426","archive":["Portico"],"relation":{},"ISSN":["1424-8832","1424-8840"],"issn-type":[{"value":"1424-8832","type":"print"},{"value":"1424-8840","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005]]}}}