{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T18:05:19Z","timestamp":1759601119888,"version":"3.40.4"},"reference-count":22,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2006,4,24]],"date-time":"2006-04-24T00:00:00Z","timestamp":1145836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,4,24]],"date-time":"2006-04-24T00:00:00Z","timestamp":1145836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Exp Nephrol"],"abstract":"<i>Background:<\/i> Previous studies found that transforming growth factor-\u03b2 (TGF-\u03b2) induces mesothelial production of connective tissue growth factor (CTGF), which may be downstream mediators of TGF-\u03b2. Since high dose TGF-\u03b2 induces apoptosis of peritoneal mesothelial cells (PMC), we study the effect of CTGF blockade in the system of TGF-\u03b2-induced PMC apoptosis. <i>Method:<\/i> We examined the effect of TGF-W in primary culture of rat peritoneal mesothelial cells (PMC). PMC apoptosis was studied by flow cytometry. The effect of CTGF was blocked by antibody and short-interfering RNA (siRNA). Expression of apoptotic gene was studied by real-time polymerase chain reaction. <i>Result:<\/i> In cultured unstimulated rat PMC, there is a low but definite incidence of spontaneous apoptosis. Stimulation with TGF-\u03b2 50 pg\/ml induces an upregulation of apoptotic gene BAX expression and a downregulation of anti-apoptotic gene BCL-2L expression, and a 4-fold increase in PMC apoptosis. The effect of TGF-\u03b2-induced PMC apoptosis was partly prevented by antibody against CTGF, and completely abolished by CTGF-specific siRNA, while CTGF-blockade by siRNA had no effect on PMC necrosis. CTGF silencing by siRNA prevented the down-regulation of BCL-2L expression induced by TGF-\u03b2, had no effect on the BAX expression. <i>Conclusion:<\/i> Our results indicate that CTGF is an important downstream mediator of TGF-\u03b2-induced PMC apoptosis.<\/jats:p>","DOI":"10.1159\/000092907","type":"journal-article","created":{"date-parts":[[2006,4,24]],"date-time":"2006-04-24T13:49:48Z","timestamp":1145886588000},"page":"e166-e174","source":"Crossref","is-referenced-by-count":14,"title":["Connective Tissue Growth Factor Is Responsible for Transforming Growth Factor-Beta-Induced Peritoneal Mesothelial Cell Apoptosis"],"prefix":"10.1159","volume":"103","author":[{"given":"Cheuk-Chun","family":"Szeto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai-Ming","family":"Chow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ka-Bik","family":"Lai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol Yi-Ki","family":"Szeto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bonnie Ching-Ha","family":"Kwan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip Kam-Tao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2006,4,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159%2F000083889"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1440-1843.2004.00602.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1093%2Fndt%2Fgfh277"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1525-1594.2003.07222.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1093%2Fndt%2Fgfg003"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00441-001-0479-6"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1097%2F01.ASN.0000033611.79556.AE"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002%2Fhep.510300503"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-5793%2899%2901051-0"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2000.00301.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.274.52.37461"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-2999%2800%2900115-1"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-2999%2899%2900763-3"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M211300200"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1159%2F000013488"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1159%2F000013397"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMoa020809"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1159%2F000013488"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016%2FS1359-6101%2897%2900010-5"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1006%2Fjmcc.2000.1215"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2003.00069.x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMra045004"}],"container-title":["Nephron Experimental Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/92907","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/92907","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:08:01Z","timestamp":1745464081000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000092907"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4,24]]},"references-count":22,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2006,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000092907","archive":["Portico"],"relation":{},"ISSN":["1660-2129"],"issn-type":[{"type":"electronic","value":"1660-2129"}],"subject":[],"published":{"date-parts":[[2006,4,24]]}}}