{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T18:29:19Z","timestamp":1786559359926,"version":"build-2736575974"},"reference-count":20,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Caries Res"],"published-print":{"date-parts":[[2006]]},"abstract":"Small salivary phosphoproteins \u2013 statherin (ST) and histatin 1 (HT1) \u2013 are found in the acquired enamel pellicle which modulates <i>Streptococcus mutans<\/i> adhesion onto dental enamel. However, their roles in <i>S. mutans<\/i> adhesion onto enamel surfaces are still undefined. The aim of this study was to investigate whether and how ST and HT1 affect (i) <i>S. mutans<\/i> adhesion and (ii) the adsorption of <i>S. mutans<\/i> adhesion-promoting salivary proteins onto hydroxyapatite (HA) in vitro. We fractionated human parotid saliva by adsorption to HA and further by gel filtration chromatography. Adhesion of [<sup>3<\/sup>H]-labeled <i>S. mutans<\/i> strain MT8148 onto sintered HA plates was promoted significantly (>10-fold) by high-molecular weight glycoprotein fraction (HMWGP), but not by purified ST or HT1. More interestingly, promotion of <i>S. mutans<\/i> adhesion onto HA by HMWGP was significantly reduced by adding purified ST or HT1 to HMWGP. [<sup>3<\/sup>H]-labeled <i>S. mutans<\/i> adhesion on HA was positively correlated to the [<sup>14<\/sup>C]-labeled HMWGP adsorption onto HA, which was also reduced by the addition of purified ST and HT1. Synthetic peptides corresponding to ST and HT1 reduced the parotid saliva-promoted <i>S. mutans<\/i> adhesion. However, removal of the negative charges in the N-terminal domains of ST and HT1 diminished their inhibitory effects on <i>S. mutans<\/i> adhesion promoted by parotid saliva. We conclude that ST and HT1 competitively inhibit the adsorption of salivary HMWGP, and thereby reduce <i>S. mutans<\/i> adhesion onto HA surfaces.<\/jats:p>","DOI":"10.1159\/000094286","type":"journal-article","created":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T11:41:59Z","timestamp":1157110919000},"page":"403-411","source":"Crossref","is-referenced-by-count":60,"title":["Statherin and Histatin 1 Reduce Parotid Saliva-Promoted <i>Streptococcus mutans<\/i> Strain MT8148 Adhesion to Hydroxyapatite Surfaces"],"prefix":"10.1159","volume":"40","author":[{"given":"A.","family":"Shimotoyodome","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Tokimitsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Matsukubo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Takaesu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2006,8,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2F0003-9969%2889%2990070-8"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1177%2F10454411980090010401"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1177%2F00220345950740120601"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1177%2F00220345890680090201"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2F0003-9969%2873%2990128-3"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2F0003-9969%2875%2990073-4"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.archoralbio.2004.06.003"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2F0003-9969%2891%2990147-M"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0003-9969%2800%2900047-9"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1574-6968.1991.tb05118.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1042%2F0264-6021%3A3590243"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1177%2F10454411960070020601"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2F0003-9969%2886%2990048-8"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1159%2F000260299"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2F0020-711X%2892%2990387-G"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1600-051X.1990.tb01077.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002%2Fbmc.438"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002%2Fpmic.200300638"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1021%2Fpr049911o"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M206333200"}],"container-title":["Caries Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/94286","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/94286","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:30:16Z","timestamp":1745461816000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000094286"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006]]},"references-count":20,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2006,8,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000094286","archive":["Portico"],"relation":{},"ISSN":["0008-6568","1421-976X"],"issn-type":[{"value":"0008-6568","type":"print"},{"value":"1421-976X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006]]}}}