{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:57:39Z","timestamp":1745470659132,"version":"3.40.4"},"reference-count":10,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[2006]]},"abstract":"<jats:p>Previous studies have shown that T lymphocyte proliferation and apoptosis are abnormal in idiopathic thrombocytopenic purpura (ITP) children; however, the underlying regulated mechanisms in signal transductions remain unknown. In this paper, we investigated the changes of protein kinase C (PKC) activity in peripheral blood T lymphocytes and the effect of PKC on the peripheral blood T lymphocyte proliferation and apoptosis in ITP children. We demonstrated that T lymphocytes from ITP children were more susceptible to the activator (phorbol myristate acetate) and the inhibitor (H-7) of PKC. In ITP children, phorbol myristate acetate and H-7 dramatically affected T lymphocyte proliferation and apoptosis, but altered little in healthy children. Compared with healthy children, PKC activity was significantly enhanced in ITP children, increasing the expressions of Fas ligand on CD3+, CD4+ and CD8+ T cells, indicating positive correlations between PKC activity and the expressions of Fas ligand on T cells, while the relations between PKC activity and platelet count showed negative correlations. Taken together, our findings suggest that PKC signal transductions may participate in the procedure of T lymphocyte proliferation and apoptosis in ITP children. PKC activation may enhance T lymphocyte activity, suppress T cell apoptosis, and be involved in thrombocyte damage, which can be related to the immunity pathogenesis of ITP.<\/jats:p>","DOI":"10.1159\/000094677","type":"journal-article","created":{"date-parts":[[2006,9,29]],"date-time":"2006-09-29T13:05:50Z","timestamp":1159535150000},"page":"173-180","source":"Crossref","is-referenced-by-count":1,"title":["Effect of Protein Kinase C on T Lymphocyte Proliferation and Apoptosis in Acute Idiopathic Thrombocytopenic Purpura"],"prefix":"10.1159","volume":"116","author":[{"given":"Fang","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-lin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-meng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue-wen","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2006,10,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1054%2Fblre.2001.0177"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M104919200"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007%2FPL00012236"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2F0090-1229%2891%2990065-I"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002%2Feji.1830270622"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.immunol.20.100301.064807"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038%2Fnm921"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1600-0609.2000.9o096.x"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.cr.7290074"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.0022-202X.2003.22109.x"}],"container-title":["Acta Haematologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/94677","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/94677","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:34:19Z","timestamp":1745462059000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000094677"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006]]},"references-count":10,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2006,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000094677","archive":["Portico"],"relation":{},"ISSN":["0001-5792","1421-9662"],"issn-type":[{"type":"print","value":"0001-5792"},{"type":"electronic","value":"1421-9662"}],"subject":[],"published":{"date-parts":[[2006]]}}}