{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:00:04Z","timestamp":1790625604039,"version":"4.1.0"},"reference-count":20,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytogenet Genome Res"],"published-print":{"date-parts":[[2006]]},"abstract":"<jats:p>Taste receptor family 1 member 3, TAS1R3, is shown to be involved in sweet and umami tastes in mouse, and the nucleotide sequence of the gene has been reported in rat, gorilla, and human. Pigs are frequently used as models for human diseases, and are also considered to be source animals for xenotransplantation to humans due to their anatomical and physiological similarities to humans. Therefore, in the present study, the genomic structure of the swine &lt;i&gt;TAS1R3&lt;\/i&gt; gene was determined, and &lt;i&gt;TAS1R3&lt;\/i&gt; expression was studied in various swine tissues. The gene was shown to reside on swine chromosome 6q22\u2192q23, from which three types of mRNAs were generated: 3,752 bp derived from six exons in tongue, 3,704 bp from six exons and 3,630 bp from seven exons in testis. The 6 exons\/5 introns were structurally similar to those of humans and mice, but the 7 exons\/6 introns structure of &lt;i&gt;TAS1R3&lt;\/i&gt; was first observed in swine. High expressions of &lt;i&gt;TAS1R3&lt;\/i&gt; were revealed in tongue, kidney, and testis by real-time PCR. The expression profile of the tissues except for kidney was similar to that of mouse. When in situ hybridization using an RNA probe for &lt;i&gt;TAS1R3&lt;\/i&gt; was performed on swine tongue and testis tissues, &lt;i&gt;TAS1R3&lt;\/i&gt; expressions were revealed in tongue circumvallate papillae, fungiform papillae, mucosal epithelium, follicular B lymphocytes, lymphocytes in submucosal tissues of lingual tonsil, and spermatogenic cells. Using peripheral mature B lymphocytes, the expression of &lt;i&gt;TAS1R3 &lt;\/i&gt;in B lymphocytes was further confirmed by real-time PCR and sequencing of the real-time PCR product.<\/jats:p>","DOI":"10.1159\/000094801","type":"journal-article","created":{"date-parts":[[2006,9,15]],"date-time":"2006-09-15T08:19:38Z","timestamp":1158308378000},"page":"51-61","source":"Crossref","is-referenced-by-count":45,"title":["Genomic structure of swine taste receptor family 1 member 3, &lt;i&gt;TAS1R3&lt;\/i&gt;, and its expression in tissues"],"prefix":"10.1159","volume":"115","author":[{"given":"S.","family":"Kiuchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Kiyokawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Saito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Fujimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Yasue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2006,9,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0092-8674%2800%2980705-9"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159%2F000133931"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.84.24.8869"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1085%2Fjgp.100.5.803"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2FS0959-4388%2800%2900118-5"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.2001.4760"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.072090199"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1152%2Fjn.01198.2003"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038%2F35093032"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038%2F88270"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2052.1999.00493.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1093%2Fbioinformatics%2F16.6.558"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS0092-8674%2801%2900451-2"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038%2Fnature726"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007%2Fs002239900491"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1523%2FJNEUROSCI.1374-03.2004"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2052.2000.00588.x"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2F0042-6822%2882%2990406-8"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1159%2F000015095"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2FS0092-8674%2803%2900844-4"}],"container-title":["Cytogenetic and Genome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/94801","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/94801","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:35:29Z","timestamp":1745462129000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000094801"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2006,9,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000094801","archive":["Portico"],"relation":{},"ISSN":["1424-8581","1424-859X"],"issn-type":[{"value":"1424-8581","type":"print"},{"value":"1424-859X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006]]}}}