{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T16:59:07Z","timestamp":1761843547002,"version":"3.40.4"},"reference-count":23,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2006]]},"abstract":"<i>Background:<\/i> Prostaglandin D<sub>2<\/sub> (PGD<sub>2<\/sub>), a major prostanoid produced by activated mast cells, has long been implicated in allergic diseases. PGD<sub>2<\/sub> demonstrates its effects through two G-protein-coupled receptors, DP and CRTH2. The PGD<sub>2<\/sub>\/CRTH2 system mediates chemotaxis of eosinophils, basophils, and Th2 cells, which are involved in the induction of allergic inflammation. Although recent studies have shown that the specific receptors for PGD<sub>2<\/sub>, DP, and CRTH2 are expressed in various human tissues, the role of PGD<sub>2<\/sub> is unknown in human bronchial epithelial cells. In this study, we investigated the expression and function of CRTH2\/DP on NCI-H<sub>292<\/sub> and NHBE cells. <i>Method:<\/i> The CRTH2\/DP expression was examined by RT-PCR and flow-cytometric analysis. NCI-H<sub>292<\/sub> and NHBE cells were cultured in the presence of various stimulants. The resulting supernatants were measured by ELISA. <i>Results:<\/i> We demonstrated that PGD<sub>2<\/sub> induced production of IL-8 and GM-CSF in NCI-H<sub>292<\/sub> and NHBE cells. DK-PGD<sub>2<\/sub> (CRTH2 agonist) and latanoprost (FP, a prostaglandin F receptor, agonist) failed to augment the production of these cytokines. Pretreatment with ramatroban (CRTH2 antagonist) and AL8810 (FP antagonist) did not reduce the production of these cytokines. The PGD<sub>2<\/sub>-induced cytokine production was inhibited by pertussis toxin or specific inhibitors for MAP\/ERK kinase (PD98059) and p38 MAP kinase (SB202190). <i>Conclusion:<\/i> These results suggest that PGD<sub>2<\/sub> is a potent inducer of IL-8 and GM-CSF production with MAP\/ERK and p38 MAP kinase activation, but this is independent of CRTH2 activation.<\/jats:p>","DOI":"10.1159\/000095436","type":"journal-article","created":{"date-parts":[[2006,8,28]],"date-time":"2006-08-28T13:59:38Z","timestamp":1156773578000},"page":"300-307","source":"Crossref","is-referenced-by-count":17,"title":["Prostaglandin D<sub>2<\/sub> Induces IL-8 and GM-CSF by Bronchial Epithelial Cells in a CRTH2-Independent Pathway"],"prefix":"10.1159","volume":"141","author":[{"given":"Takahito","family":"Chiba","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Kanda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeharu","family":"Ueki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wataru","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Kamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajime","family":"Oyamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norihiro","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Kayaba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junichi","family":"Chihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2006,10,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2899%2970281-2"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1183%2F09031936.94.07122226"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM198407263110401"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.91.23.11192"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0702392"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0702393"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.287.5460.2013"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0704973"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1747.2002.01862.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1152%2Fajplung.00313.2004"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-5793%2899%2901251-X"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.1993.tb00295.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1183%2F09031936.94.07122109"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.pharmtox.41.1.661"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2FS0952-3278%2803%2900078-4"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.270.32.18910"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.bbrc.2004.02.151"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.193.2.255"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016%2FS0006-2952%2898%2900059-8"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2898%2970311-2"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.2000.00641.x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1002%2Feji.200323330"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016%2FS0090-6980%2801%2900155-1"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/95436","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/95436","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:41:23Z","timestamp":1745462483000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000095436"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006]]},"references-count":23,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2006,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000095436","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2006]]}}}