{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T19:48:32Z","timestamp":1762458512442,"version":"3.40.4"},"reference-count":26,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Digestion"],"published-print":{"date-parts":[[2006]]},"abstract":"<i>Aim:<\/i> To investigate the functional and molecular mechanisms by which sulfated cholecystokinin octapeptide (CCK-8S) regulates calcium mobilization in gastric antral smooth muscle cells (SMCs) of rats. <i>Methods:<\/i> Isotonic contraction of antral strips was recorded using a polyphysiograph. Immunoprecipitation was used to determine the regulatory effect of protein kinase C (PKC) on regulating the phosphorylation of the type III inositol 1,4,5-triphosphate receptor (InsP<sub>3<\/sub>R3) in gastric SMCs. Alterations in the intracellular calcium ([Ca<sup>2+<\/sup>]<sub>i<\/sub>) concentration were assayed using fura-2\/AM-loaded microspectrofluorometry, and the L-type calcium current (I<sub>Ca-L<\/sub>) was recorded by patch-clamp techniques. <i>Results:<\/i> CCK-8S (5 \u00d7 10<sup>\u20138<\/sup> mol\/l) significantly increased the mean contractile amplitude of circular muscle by 61.85 \u00b1 12.67% and the frequency of longitudinal muscle by 57.91 \u00b1 15.70% in gastric antral strips, which were suppressed by dexloxiglumide or thapsigargin (TG) and BAPTA-AM (BA). Treatment withchelerythrine (5 \u00d7 10<sup>\u20138<\/sup> mmol\/l) significantly inhibited the CCK-8S-increased phosphorylation of InsP<sub>3<\/sub>R3 in SMCs. The amplitudes of the CCK-8S-triggered [Ca<sup>2+<\/sup>]<sub>i<\/sub> concentration oscillations were reduced in a dose-dependent manner when the SMCs were pretreated with increasing concentrations of PMA (from 10<sup>\u20138<\/sup> to 10<sup>\u20135<\/sup> mol\/l). On removal of extracellular calcium or blocking I<sub>Ca-L<\/sub> by nifedipine, a smaller but significant rise in the [Ca<sup>2+<\/sup>]<sub>i<\/sub> concentration was still elicited by CCK-8S. When [Ca<sup>2+<\/sup>]<sub>i<\/sub> was depleted by the administration of 10<sup>\u20135<\/sup> mol\/l TG and 10<sup>\u20135<\/sup> mol\/l BA or blocked by the calcium-dependent chloride current (I<sub>Cl-Ca<\/sub>) by giving 5 \u00d7 10<sup>\u20136<\/sup> mol\/l niflumic acid, the CCK-8S-intensified I<sub>Ca-L<\/sub> (from \u201356.42 \u00b1 6.57 to \u201388.54 \u00b1 5.71 pA) was apparently inhibited by 90.34 \u00b1 4.71% and 82.59 \u00b1 4.24%. <i>Conclusions:<\/i> These results demonstrate that the CCK-8S-evoked [Ca<sup>2+<\/sup>]<sub>i<\/sub> concentration increase in gastric antral SMCs depends on the release of [Ca<sup>2+<\/sup>]<sub>i<\/sub> stores which are negatively regulated by PKC-mediated phosphorylation of InsP<sub>3<\/sub>R3. Released calcium in turn activates I<sub>Ca-L<\/sub> through the activation of I<sub>Cl-Ca<\/sub>, ultimately resulting in the contraction of the gastric smooth muscle.<\/jats:p>","DOI":"10.1159\/000095628","type":"journal-article","created":{"date-parts":[[2006,9,6]],"date-time":"2006-09-06T13:09:10Z","timestamp":1157548150000},"page":"249-258","source":"Crossref","is-referenced-by-count":5,"title":["Signal Transduction Pathways Mediating CCK-8S-Induced Gastric Antral Smooth Muscle Contraction"],"prefix":"10.1159","volume":"73","author":[{"given":"Xin-Min","family":"Si","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley Chireyath","family":"Paul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"He-Sheng","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2006,9,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1152%2Fajpgi.00057.2003"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002%2Fmed.10042"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2F0014-2999%2896%2900213-0"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0705769"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M204318200"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1113%2Fjphysiol.2002.027904"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0196-9781%2802%2900136-5"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1085%2Fjgp.116.4.547"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M313476200"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.273.33.21267"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1254%2Fjphs.FP0050387"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0928-4257%2801%2900053-5"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007%2Fs004410100402"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1152%2Fphysrev.00004.2004"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2826.2005.01312.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.2003-0176"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1038%2F35036035"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2FS0960-9822%2801%2900326-8"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M004276200"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M104308200"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1469-7793.2000.00105.x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M308378200"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016%2FS0006-291X%2803%2900616-8"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.284.5419.1527"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1152%2Fajpgi.00291.2003"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0705584"}],"container-title":["Digestion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/95628","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/95628","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:43:48Z","timestamp":1745462628000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000095628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006]]},"references-count":26,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2006,9,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000095628","archive":["Portico"],"relation":{},"ISSN":["0012-2823","1421-9867"],"issn-type":[{"type":"print","value":"0012-2823"},{"type":"electronic","value":"1421-9867"}],"subject":[],"published":{"date-parts":[[2006]]}}}