{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T05:29:30Z","timestamp":1761974970339,"version":"3.40.4"},"reference-count":20,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2006,9,29]],"date-time":"2006-09-29T00:00:00Z","timestamp":1159488000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,9,29]],"date-time":"2006-09-29T00:00:00Z","timestamp":1159488000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2007]]},"abstract":"<i>Background:<\/i> Ash tree <i>(Fraxinus excelsior)<\/i> is the main representative of the Oleaceae family in temperate zones. Diagnosis of ash pollen allergy is made difficult due to (1) an overlapping pollinization period with Betulaceae, (2) non-inclusion in current diagnostic assays, and (3) some cross- reactivity with minor allergens from Betulaceae. The aim of this study was to calibrate an ash pollen in-house reference preparation (IHRP) in allergic patients in order to produce standardized products for diagnosis and immunotherapy purposes. <i>Methods:<\/i> Ash pollen IHRP was extracted, ultrafiltered and freeze dried. Allergens in the extract were detected after 2-dimensional PAGE using specific sera and a monoclonal antibody. The Fra e 1 content of IHRP was evaluated by quantitative immunoprint. Forty-eight subjects from the North-East of France exhibiting clinical symptoms, a positive skin test and specific IgE levels \u2267class 2 to ash pollen were recruited. IgE immunoprints were performed to select patients sensitized to the ash Fra e 1 allergen as opposed to cross-reacting allergens. Serial 10-fold dilutions of the IHRP were tested by skin prick tests in order to determine the concentration inducing a geometrical mean wheal diameter of 7 mm, said to correspond to an index of reactivity (IR) of 100 per millilitre. <i>Results:<\/i> IgE-reactive molecules in IHRP comprise Fra e 1, Fra e 2, a 9-kDa molecule (presumably Fra e 3), as well as a doublet at 15 kDa and high molecular weight allergens. The 100 IR concentration of IHRP inducing a geometrical mean wheal diameter of 7 mm in 22 patients sensitized to Fra e 1 corresponds to the 1\/126 (w\/v) extraction ratio (i.e. 259 \u00b5g\/ml of protein by Bradford) and contains 17 \u00b5g\/ml of Fra e 1. The variability in total activity of 5 batches of standardized extracts was found to be significantly reduced when compared with 7 non-standardized extracts. <i>Conclusion:<\/i> An ash pollen IHRP was defined and molecularly characterized. Its successful standardization at 100 IR\/ml in patients specifically sensitized to Fra e 1 allowed a skin reactivity-based calibration in properly diagnosed patients. Such a standardized ash pollen extract is a reliable tool to support immunotherapy of ash pollen allergy.<\/jats:p>","DOI":"10.1159\/000095994","type":"journal-article","created":{"date-parts":[[2006,9,29]],"date-time":"2006-09-29T13:05:50Z","timestamp":1159535150000},"page":"11-18","source":"Crossref","is-referenced-by-count":18,"title":["Standardization of an Ash <i>(Fraxinus excelsior) <\/i>Pollen Allergen Extract"],"prefix":"10.1159","volume":"142","author":[{"given":"Maud","family":"Hrabina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashok","family":"Purohit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Philippe","family":"Oster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioanna","family":"Papanikolaou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine","family":"Jain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poncet","family":"Pascal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hubert","family":"Sicard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"Gouyon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Moingeon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabrielle","family":"Pauli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Andr\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2006,9,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.1988.tb02905.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159%2F000237370"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaci.2004.10.001"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.2002.01369.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1398-9995.2000.00671.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.1985.tb02293.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2896%2970231-2"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1398-9995.1997.tb02154.x"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.1993.tb00328.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1398-9995.2002.057s71029.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1159%2F000236725"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1432-1033.1993.tb18208.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1398-9995.1998.tb04090.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2004.02186.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007%2Fs11882-001-0036-0"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1398-9995.2003.00247.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1398-9995.1997.tb02544.x"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.1979.tb00484.x"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1159%2F000057742"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1159%2F000082332"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/95994","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/95994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:47:59Z","timestamp":1745462879000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000095994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9,29]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2006,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000095994","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2006,9,29]]}}}