{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T09:52:44Z","timestamp":1760608364647,"version":"3.40.4"},"reference-count":29,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2006,11,22]],"date-time":"2006-11-22T00:00:00Z","timestamp":1164153600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,11,22]],"date-time":"2006-11-22T00:00:00Z","timestamp":1164153600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2007]]},"abstract":"<i>Background:<\/i> Allergy is an immunological disorder affecting about 25% of the population living in the industrialized countries. Specific immunotherapy is the only treatment with a long-lasting relief of allergic symptoms and able to reduce the risk of developing new allergic sensitizations and inhibiting the development of clinical asthma in children treated for allergic rhinitis. <i>Methods:<\/i> By means of DNA recombinant technology, we were able to design a head to tail dimer expressing disulphide bond variants of the major allergen of the <i>Parietaria <\/i>pollen. IgE binding activity was studied by Western blot, ELISA inhibition assays and the skin prick test. T cell recognition was studied by peripheral blood mononuclear cell proliferation. The immunogenicity of the hybrid was studied in a mouse model of sensitization. <i>Results:<\/i> In vitro and in vivo analysis showed that the disruption of specific cysteine residues in both allergens caused a strong reduction in IgE binding activity of the PjEDcys hybrid. In addition,we were able to show that a reduction in the IgE epitope content profoundly reduced the anaphylactic activity of the hybrid (from 100 to 1,000 times less than wild-type allergens) without interfering with the T cell recognition. Sera from BALB\/c mice immunized with the hybrid were able to bind the natural <i>Parietaria<\/i> allergens and to inhibit the binding of human IgE to wild-type Par j 1 and Par j 2 allergens up to 90%. <i>Conclusion:<\/i> Our results demonstrate that hybrid-expressing disulphide bond variants of the major allergens of the <i>Parietaria<\/i> pollen displayed reduced allergenicity and maintained T cell reactivity for induction of protective antibodies.<\/jats:p>","DOI":"10.1159\/000097358","type":"journal-article","created":{"date-parts":[[2006,11,29]],"date-time":"2006-11-29T17:37:54Z","timestamp":1164821874000},"page":"274-284","source":"Crossref","is-referenced-by-count":19,"title":["A Hybrid Expressing Genetically Engineered Major Allergens of the <i>Parietaria<\/i> Pollen as a Tool for Specific Allergy Vaccination"],"prefix":"10.1159","volume":"142","author":[{"given":"A.","family":"Bonura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Corinti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Artale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Di 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