{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:43:14Z","timestamp":1761154994892,"version":"3.40.4"},"reference-count":18,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2006,11,30]],"date-time":"2006-11-30T00:00:00Z","timestamp":1164844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,11,30]],"date-time":"2006-11-30T00:00:00Z","timestamp":1164844800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intervirology"],"published-print":{"date-parts":[[2007]]},"abstract":"<jats:p>&lt;i&gt;Objective:&lt;\/i&gt; We have compared the gene expression and DNA immunization efficacy encoding prME and E proteins of a different strain (JaGAr-01) derived from Japanese encephalitis virus. This study aimed to construct a recombinant encoding E protein of the Beijing-1 strain derived from Japanese encephalitis virus and analyze the humoral, cellular and protective immunity induced by the above recombinant. &lt;i&gt;Methods:&lt;\/i&gt; The recombinant pJBE containing E (1,500 bps) gene from the Beijing-1 strain of Japanese encephalitis virus was constructed and then transfected into the HepG2 cell line by liposome fusion. The expression of E (about 53 kD) protein in transfected cells was analyzed by Western blot using a specific anti-JEV-E antibody. BALB\/c mice were vaccinated with 3 \u00b5g of pJBE by the gene-gun technique. JaGAr-01 and Beijing-1 strains (10&lt;sup&gt;5&lt;\/sup&gt; PFU\/100 \u00b5l) of Japanese encephalitis virus were given to BALB\/c mice by intraperitoneal injection 3 weeks after double DNA immunization with a lethal virus challenge. BALB\/c mice were observed for 21 days after challenge. An 80% plaque reduction neutralization test was performed to titrate the neutralization antibody before and after viral challenge. A lactate dehydrogenase activity release test was used to examine cytotoxic T lymphocyte activity after double DNA immunization. &lt;i&gt;Results:&lt;\/i&gt; The expression of about 53 kD protein associated with pJBE was determined in transfected HepG2 cells with specific anti-JEV-E antibody. A higher level of neutralization antibodies and the cytotoxicity effect were induced with pJBE immunization using the gene-gun technique, and were similar to those induced with inactivated vaccine derive from the Beijing-1 strain of Japanese encephalitis virus. Balb\/c mice immunized with pJBE survived the challenge with the different strains of Japanese encephalitis virus; however, Balb\/c mice immunized with inactivated vaccine did not survive the challenge with the JaGAr-01 strain of Japanese encephalitis virus at all. &lt;i&gt;Conclusions:&lt;\/i&gt; DNA vaccine containing the E protein gene derived from Japanese encephalitis virus can provide not only better efficacy including humoral and cellular immunity, but also cross-protection against infection with homologous and heterologous Japanese encephalitis virus.<\/jats:p>","DOI":"10.1159\/000097395","type":"journal-article","created":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T12:39:49Z","timestamp":1164976789000},"page":"93-98","source":"Crossref","is-referenced-by-count":8,"title":["Immunologic Analysis Induced by DNA Vaccine Encoding E Protein of Beijing-1 Strain Derived from Japanese Encephalitis Virus"],"prefix":"10.1159","volume":"50","author":[{"given":"Guo-he","family":"Feng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-zhen","family":"Zhai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi-mei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-guang","family":"Dou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2006,11,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1136%2Fjnnp.68.4.405"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2FS0264-410X%2800%2900037-2"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1749-6632.2001.tb02702.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2FS0264-410X%2801%2900208-0"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1586%2F14760584.2.3.407"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.120162497"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.8456302"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2F0042-6822%2892%2990526-U"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1586%2F14760584.3.2.199"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00572709"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01702653"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1128%2FJVI.75.5.2204-2212.2001"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.90.9.4156"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1093%2Fhmg%2F2.11.1847"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1006%2Fviro.1996.0603"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1099%2F0022-1317-77-4-705"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002%2Fjmv.1890440204"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1017%2FS095026889700753X"}],"container-title":["Intervirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/97395","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/97395","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:03:11Z","timestamp":1745463791000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000097395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11,30]]},"references-count":18,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2007,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000097395","archive":["Portico"],"relation":{},"ISSN":["0300-5526","1423-0100"],"issn-type":[{"type":"print","value":"0300-5526"},{"type":"electronic","value":"1423-0100"}],"subject":[],"published":{"date-parts":[[2006,11,30]]}}}