{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:57:30Z","timestamp":1745470650596,"version":"3.40.4"},"reference-count":19,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemotherapy"],"published-print":{"date-parts":[[2007]]},"abstract":"<i>Background:<\/i> Activity of gatifloxacin against clinical isolates of fluoroquinolone-resistant <i>Staphylococcus aureus<\/i> is more potent than that of other fluoroquinolones such as norfloxacin and levofloxacin. To date, few reports have described high-level resistance to gatifloxacin in clinical isolates of <i>S. aureus,<\/i> although in vitro studies have shown that mutations in both DNA gyrase and topoisomerase IV were required for gatifloxacin resistance in <i>S. aureus.<\/i><i>Methods:<\/i> Minimum inhibitory concentrations were determined for fluoroquinolones and other antimicrobials in a methicillin-resistant <i>S. aureus<\/i> isolate that was cultured from blood of a patient with septicemia. Fluoroquinolone resistance was characterized by DNA sequencing and microbiologic assay. <i>Results:<\/i> The isolate showed high-level resistance to fluoroquinolones including an 8-methoxyfluoroquinolone, gatifloxacin (minimum inhibitory concentration 64 \u00b5g\/ml). Amino acid mutations of Ser80Tyr and Glu84Lys in GrlA and Ser84Leu and Ser85Pro in GyrA were possibly related to this resistance in methicillin-resistant <i>S. aureus<\/i> HU2000-062, although efflux may play a minor role in resistance as well. <i>Conclusion:<\/i> GyrA and GrlA mutations mainly conferred to 8-methoxyfluoroquinolone resistance in this isolate.<\/jats:p>","DOI":"10.1159\/000098427","type":"journal-article","created":{"date-parts":[[2007,1,5]],"date-time":"2007-01-05T14:48:22Z","timestamp":1168008502000},"page":"104-109","source":"Crossref","is-referenced-by-count":6,"title":["Molecular Characterization of 8-Methoxyfluoroquinolone Resistance in a Clinical Isolate of Methicillin-Resistant <i>Staphylococcus aureus<\/i>"],"prefix":"10.1159","volume":"53","author":[{"given":"Toshinobu","family":"Horii","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Takeshita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Maekawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2007,1,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.3201%2Feid0702.010239"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2FS0732-8893%2803%2900037-3"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1574-695X.1999.tb01400.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2FS0732-8893%2898%2900101-1"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2F45.4.437"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2F42.4.561"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.45.5.1553-1557.2001"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.45.10.2755-2764.2001"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkg162"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.49.8.3325-3333.2005"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1128%2FCMR.14.4.836-871.2001"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.45.12.3544-3547.2001"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1159%2F000069779"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1159%2F000085612"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.45.3.938-942.2001"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.45.12.3422-3426.2001"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2958.1994.tb00458.x"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1099%2F00222615-42-3-214"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkg241"}],"container-title":["Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/98427","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/98427","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:31:36Z","timestamp":1745461896000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000098427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007]]},"references-count":19,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2007,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000098427","archive":["Portico"],"relation":{},"ISSN":["0009-3157","1421-9794"],"issn-type":[{"type":"print","value":"0009-3157"},{"type":"electronic","value":"1421-9794"}],"subject":[],"published":{"date-parts":[[2007]]}}}