{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T10:58:02Z","timestamp":1761217082008,"version":"3.40.4"},"reference-count":37,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Vasc Res"],"published-print":{"date-parts":[[2007]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Platelet activation results in platelet aggregation and the secretion of granules, which contain a variety of constituents including nonprotein molecules, adhesive proteins and hydrolases. The platelet-derived supernatant (PDS), which contains these granules, is known to trigger the activation of endothelium and chemotaxis of monocytes. &lt;i&gt;Methods and Results:&lt;\/i&gt; PDS derived from collagen-activated platelets stimulated human umbilical vein endothelial cell (HUVEC) migration and invasion, as measured through the use of a Boyden chamber. This collagen-induced PDS also triggered integrin \u03b1&lt;sub&gt;v&lt;\/sub&gt;\u03b2&lt;sub&gt;3&lt;\/sub&gt; upregulation in HUVECs. The inclusion of a neutralizing antibody to platelet-derived growth factor (PDGF)-B abolished HUVEC migration\/invasion and integrin \u03b1&lt;sub&gt;v&lt;\/sub&gt;\u03b2&lt;sub&gt;3&lt;\/sub&gt; upregulation, showing that PDGF-AB mediates the proangiogenic effects of collagen-activated PDS. Saxatilin, a snake venom disintegrin known to interrupt platelet aggregation by antagonizing integrin \u03b1&lt;sub&gt;IIb&lt;\/sub&gt;\u03b2&lt;sub&gt;3&lt;\/sub&gt;, inhibited the collagen-induced platelet activation and abolished the angiogenic properties of PDS. Saxatilin also inhibited the collagen-induced phosphorylation of Syk, a key mediator of inside-out signaling in platelet activation. &lt;i&gt;Conclusion:&lt;\/i&gt; Saxatilin inhibits platelet activation, platelet PDGF-AB release as well as subsequent endothelial cell migration and invasion.<\/jats:p>","DOI":"10.1159\/000098519","type":"journal-article","created":{"date-parts":[[2007,1,11]],"date-time":"2007-01-11T15:36:00Z","timestamp":1168529760000},"page":"129-137","source":"Crossref","is-referenced-by-count":12,"title":["Saxatilin, a Snake Venom Disintegrin, Regulates Platelet Activation Associated with Human Vascular Endothelial Cell Migration and Invasion"],"prefix":"10.1159","volume":"44","author":[{"given":"Yoon-Jung","family":"Jang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ok-Hee","family":"Jeon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doo-Sik","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2007,1,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.thromres.2004.07.020"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1242%2Fjcs.01325"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1538-7836.2003.00304.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1161%2F01.ATV.0000095974.95122.EC"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1161%2F01.RES.0000160077.17427.57"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2898%2905095-8"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1002%2Fijc.20657"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0301-472X%2802%2900791-9"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1080%2F09537100310001643999"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cardiores.2005.04.007"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007%2F978-3-642-59953-8_1"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.141.3.805"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1038%2F35025215"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.140.5.1255"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.200304105"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.7512751"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS0049-3848%2898%2900053-X"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2FS0049-3848%2801%2900416-9"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016%2FS0006-291X%2802%2900258-9"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1006%2Fabio.1994.1066"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1006%2Fexer.2001.1161"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1078%2F0171-9335-00113"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1080%2F09537100120068170"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2F1357-2725%2895%2900156-5"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1093%2Femboj%2F16.18.5600"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.bbrc.2003.08.049"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1080%2F09537100500163226"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1016%2FS0006-2952%2802%2900976-0"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1538-7836.2003.00136.x"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1016%2FS0002-8703%2803%2900368-5"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1016%2FS0049-3848%2899%2900155-3"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cellsig.2004.05.008"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1016%2FS1357-2725%2896%2900122-7"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1128%2FMCB.23.14.4764-4777.2003"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M004068200"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.200208043"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1093%2Femboj%2F16.9.2333"}],"container-title":["Journal 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