{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:48:32Z","timestamp":1792100912297,"version":"4.4.0"},"reference-count":27,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Pharmacol Physiol"],"published-print":{"date-parts":[[2007]]},"abstract":"<jats:p>The tissue cultures of explants of neonatal rat peritoneum have been demonstrated to be a sensitive test for tissue compatibility with wound antiseptics. The present study investigated the suitability of this method to assess the relative toxicity of mouthrinses to tissue. Mouthrinses containing 0.1% chlorhexidine (Chlorhexamed\u00ae Fluid 0.1%) (A), 0.3% triclosan (Colgate\u00ae) (B), essential oil in ethanolic solution (Listerine\u00ae) (C), and amine\/stannous fluoride (Meridol\u00ae) (D) were tested at use concentration and in dilutions of 10, 1, and 0.1% with exposure times of 1, 10, and 30 min, respectively. The mouthrinses (test) and Ringer\u2019s solutions (control) were applied to opened rat peritoneum. After thorough irrigation with Ringer\u2019s solution, a piece of peritoneum was removed and 1 \u00d7 1 mm explants were cut. The explants were cultivated with a bovine serum culture medium in 24-well plates at 37\u00b0C in a CO&lt;sub&gt;2&lt;\/sub&gt; incubator (95% air, 5% CO&lt;sub&gt;2&lt;\/sub&gt;). After 10 days, the tissue proliferation for the explants was assessed by a stereo microscope at 10\u00d7 magnification after ethanol fixing and hemalaun staining. With 24 grafts per test, the proliferation rate was calculated relative to a control, which was run for each mouthrinse and concentration\/time combination. Data were analyzed using ANOVA (SPSS 11.0) and post-hoc paired t test. Statistical significance of all correlations was tested by setting the significance level at p &lt; 0.05. At most concentrations, D caused significantly less tissue damage than A or B. There was no difference between C and A or C and B at 100%. However, the toxicity of C was significantly less than A or B at 10, 1, and 0.1%. C and D behaved similarly except for the 10% (30 min) and the 1% (10 min) solutions in which C was significantly less toxic. We concluded that the rat peritoneum explant test was demonstrated to be a sensitive test to assess the relative toxicity of mouthrinses to tissue.<\/jats:p>","DOI":"10.1159\/000098703","type":"journal-article","created":{"date-parts":[[2007,1,17]],"date-time":"2007-01-17T10:15:49Z","timestamp":1169028949000},"page":"162-166","source":"Crossref","is-referenced-by-count":15,"title":["The Peritoneal Explant Test for Evaluating Tissue Tolerance to Mouthrinses"],"prefix":"10.1159","volume":"20","author":[{"given":"A.","family":"Welk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rosin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"L\u00fcdtke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Schwahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Kramer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Daeschlein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2007,1,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bdj.4805302"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1053%2Fjhin.2002.1391"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1378%2Fchest.109.6.1556"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1600-051X.1990.tb01092.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1600-051X.30.s5.5.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1600-051X.2002.290503.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1600-051X.2005.00702.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1600-051X.2001.281207.x"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0195-6701%2803%2900260-3"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.0909-8836.1998.eos106204.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1902%2Fjop.1999.70.12.1443"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00784-003-0226-3"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1600-0714.2004.00228.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-827X%2803%2900048-X"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.279.5349.349"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1007%2Fs001040050498"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1600-051X.1987.tb01534.x"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1601-0825.1998.tb00266.x"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1600-051X.1994.tb00313.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1600-051X.2002.290412.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1600-051X.1991.tb02316.x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1834-7819.2001.tb00270.x"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016%2FS0939-6411%2899%2900041-7"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.2042-7158.1987.tb03425.x"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016%2FS0278-6915%2899%2900164-7"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1124%2Fdmd.104.000273"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1007%2Fs002280050601"}],"container-title":["Skin Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/98703","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/98703","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:34:10Z","timestamp":1745462050000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000098703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007]]},"references-count":27,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2007,5,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000098703","archive":["Portico"],"relation":{},"ISSN":["1660-5527","1660-5535"],"issn-type":[{"value":"1660-5527","type":"print"},{"value":"1660-5535","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007]]}}}