{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T10:11:42Z","timestamp":1791022302726,"version":"4.1.0"},"reference-count":18,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respiration"],"published-print":{"date-parts":[[2007]]},"abstract":"<i>Background:<\/i>House dust mites (HDM) have been shown to be important sources of indoor allergens associated with asthma and other allergic conditions. While exogenous proteases from allergens have a direct proinflammatory role in the respiratory tract, the precise mechanisms underlying the release of cytokines from the respiratory epithelium are unclear. <i>Objectives:<\/i> The present study examines that extracellular signal-regulated kinase (ERK) activated downstream of the Ca<sup>2+<\/sup>-sensitive tyrosine kinase plays an important role in the efficient activation of the HDM-induced IL-8 signaling pathway. <i>Methods:<\/i> We examined the effect of HDM, and the role of the Ca<sup>2+<\/sup>\/calmodulin system and mitogen-activated protein kinases, on IL-8 expression in human lung epithelial cells. <i>Results:<\/i> In H292 cells, HDM induced IL-8 release in a time- and\/or dose-dependent manner. This IL-8 release was abolished by treatment with intracellular Ca<sup>2+<\/sup> chelator (BAPTA-AM), but not by EGTA or nifedipine. Calmodulin inhibitor (calmidazolium) and tyrosine kinase inhibitor (genistein) almost completely blocked IL-8 release by HDM. PD98059, an ERK pathway inhibitor, completely abolished HDM-induced IL-8 release. Moreover, PD98059, BAPTA-AM, calmidazolium and genistein suppressed the HDM-induced ERK phosphorylation. <i>Conclusions:<\/i> HDM-induced IL-8 production is predominantly regulated by Ca<sup>2+<\/sup>\/calmodulin signaling, and ERK plays an important role in signal transmission for efficient activation of the HDM-induced IL-8 signaling pathway.<\/jats:p>","DOI":"10.1159\/000099264","type":"journal-article","created":{"date-parts":[[2007,2,3]],"date-time":"2007-02-03T06:39:57Z","timestamp":1170484797000},"page":"447-453","source":"Crossref","is-referenced-by-count":20,"title":["Calcium-Calmodulin Mediates House Dust Mite-Induced ERK Activation and IL-8 Production in Human Respiratory Epithelial Cells"],"prefix":"10.1159","volume":"74","author":[{"given":"Myung Hyun","family":"Sohn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung Eun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Won","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun-Soo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Young","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyu-Earn","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2007,1,31]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2897%2970292-6"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.3347%2Fkjp.1997.35.1.9"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.1997.tb00667.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2F0091-6749%2892%2990223-O"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2F0091-6749%2893%2990120-5"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.1997.6997"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1186%2F1476-7961-1-1"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.bi.61.070192.003015"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159%2F000093932"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159%2F000091646"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaci.2004.07.060"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.148.6.1267"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.271.36.22003"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.92.20.9151"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M507140200"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1067%2Fmai.2003.1332"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1165%2Frcmb.2004-0231OC"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaci.2003.11.026"}],"container-title":["Respiration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/99264","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/99264","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:39:17Z","timestamp":1745462357000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000099264"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007]]},"references-count":18,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2007,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000099264","archive":["Portico"],"relation":{},"ISSN":["0025-7931","1423-0356"],"issn-type":[{"value":"0025-7931","type":"print"},{"value":"1423-0356","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007]]}}}