{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T10:35:46Z","timestamp":1767522946800,"version":"3.40.4"},"reference-count":24,"publisher":"S. Karger AG","issue":"1-2","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oncology"],"published-print":{"date-parts":[[2006]]},"abstract":"<i>Objective:<\/i> Our aim is to assess the clinicopathological significance of E-cadherin and \u03b2-catenin expression, as well as their association with apoptosis in gallbladder cancers. <i>Methods:<\/i> The expression of E-cadherin and \u03b2-catenin proteins was examined in 4 biliary tract cancer cell lines and 49 gallbladder cancer specimens by immunofluorescent or immunohistochemical methods and Western blotting. The apoptotic status was evaluated in the cell lines by poly(ADP-ribose) polymerase Western blotting and in the tumors by the TdT-mediated dUTP nick end labeling assay. <i>Results: <\/i>Expression of poly(ADP-ribose) polymerase (apoptosis) was only seen in cell lines that expressed both E-cadherin and \u03b2-catenin. Reduced expression of E-cadherin and \u03b2-catenin was frequently seen in advanced gallbladder cancer cases (61 and 83%, respectively) relative to pT1 cases (25 and 63%, respectively). The 5-year survival rate in cases with reduced E-cadherin expression was 26%, significantly lower than in cases with preserved E-cadherin expression (70%; p = 0.017). Cases with reduced expression of both had lower apoptotic indices and showed a worse prognosis compared with cases with reduced expression of either E-cadherin or \u03b2-catenin (p = 0.04 and 0.049, respectively). <i>Conclusions:<\/i> The expression of E-cadherin or \u03b2-catenin frequently diminishes as the tumor progresses, and abnormalities of E-cadherin and \u03b2-catenin expression were associated with decreased apoptosis in gallbladder cancers. E-cadherin expression might be a useful prognostic marker in this tumor.<\/jats:p>","DOI":"10.1159\/000100478","type":"journal-article","created":{"date-parts":[[2017,4,18]],"date-time":"2017-04-18T21:22:18Z","timestamp":1492550538000},"page":"102-110","source":"Crossref","is-referenced-by-count":24,"title":["Frequent Occurrence of Abnormal E-Cadherin\/\u03b2-Catenin Protein Expression in Advanced Gallbladder Cancers and Its Association with Decreased Apoptosis"],"prefix":"10.1159","volume":"71","author":[{"given":"Kenro","family":"Hirata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Ajiki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taro","family":"Okazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideki","family":"Horiuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsunenori","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshikazu","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2007,4,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00295925"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00268-003-6801-6"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291097-4644%2819960616%2961%3A4%3C514%3A%3AAID-JCB4%3E3.0.CO%3B2-R"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.270.10.5549"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2FS0016-5085%2897%2970218-X"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291097-0142%2819980501%2982%3A9%3C1649%3A%3AAID-CNCR9%3E3.0.CO%3B2-5"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291097-0142%2819961115%2978%3A10%3C2101%3A%3AAID-CNCR10%3E3.0.CO%3B2-2"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097%2F00000478-200206000-00009"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002%2Fijc.10920"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1067%2Fmsy.2002.125168"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2FS0168-8278%2885%2980002-7"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002%2Fijc.20342"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1136%2Fgut.38.3.426"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038%2Fmodpathol.3880409"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002%2Fbjs.1800840217"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291097-0142%2819970301%2979%3A5%3C892%3A%3AAID-CNCR4%3E3.0.CO%3B2-E"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2168.2001.01749.x"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.humpath.2004.01.024"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002%2Fpath.1711740403"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1002%2Fhep.510270412"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.274.14.9656"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.273.27.16953"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016%2FS0092-8674%2800%2981010-7"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.118.3.703"}],"container-title":["Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/100478","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/100478","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:51:15Z","timestamp":1745463075000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000100478"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006]]},"references-count":24,"journal-issue":{"issue":"1-2","published-online":{"date-parts":[[2007,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000100478","archive":["Portico"],"relation":{},"ISSN":["0030-2414","1423-0232"],"issn-type":[{"type":"print","value":"0030-2414"},{"type":"electronic","value":"1423-0232"}],"subject":[],"published":{"date-parts":[[2006]]}}}