{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:31:42Z","timestamp":1790775102147,"version":"4.1.0"},"reference-count":18,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dermatology"],"published-print":{"date-parts":[[2007]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; A rise of CD3+TCR\u03b3\u03b4+CD28\u2013 T cells has previously been observed after anin vitro long-lasting activation or even during viral infection. &lt;i&gt;Objective:&lt;\/i&gt; The aim of this study was to investigate the expression of CD28 on lymphocytes bearing CD3\/TCR\u03b3\u03b4 receptors in cancer, i.e. cutaneous melanoma. &lt;i&gt;Methods:&lt;\/i&gt; TCR\u03b3\u03b4 lymphocytes were analysed in 41 Caucasian melanoma patients and 39 healthy individuals by flow cytometry. Patients were stratified according to the American Joint Committee on Cancer (AJCC) clinical stage. &lt;i&gt;Results:&lt;\/i&gt; The number of circulating CD3+TCR\u03b3\u03b4+ T cells was significantly increased in both AJCC stages I\u2013II and AJCC stage III patients compared with healthy individuals. This increase was mediated by an accumulation of the CD3+TCR\u03b3\u03b4+CD28\u2013 T-cell subset, which expressed a high amount of perforin both in normal individuals and melanoma patients. &lt;i&gt;Conclusion:&lt;\/i&gt; This work shows, for the first time, a rise of the cytotoxic CD3+TCR\u03b3\u03b4+CD28\u2013 T-cell population in melanoma patients, which may be important in anticancer surveillance.<\/jats:p>","DOI":"10.1159\/000100878","type":"journal-article","created":{"date-parts":[[2007,4,25]],"date-time":"2007-04-25T11:59:56Z","timestamp":1177502396000},"page":"283-288","source":"Crossref","is-referenced-by-count":14,"title":["Increased Number of Cytotoxic CD3+CD28\u2013 \u03b3\u03b4 T Cells in Peripheral Blood of Patients with Cutaneous Malignant Melanoma"],"prefix":"10.1159","volume":"214","author":[{"given":"Jos\u00e9 A.","family":"Campillo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge A.","family":"Mart\u00ednez-Escribano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"Minguela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roc\u00edo","family":"L\u00f3pez-\u00c1lvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis A.","family":"Mar\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Garc\u00eda-Alonso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armand","family":"Bensussan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Roc\u00edo","family":"\u00c1lvarez-L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2007,4,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.166.1.277"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.jid.5700003"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111%2F1523-1747.ep12499808"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.2741%2F1419"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1385%2FIR%3A29%3A1-3%3A293"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002%2Fjcp.10290"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00262-004-0517-9"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002%2Feji.1830190129"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI117654"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1006%2Fclin.1996.0066"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00251-005-0065-2"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1001%2Farchderm.141.8.1016"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1747.2003.12141.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.272.37.23211"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1158%2F1078-0432.CCR-06-0019"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2FS0165-2478%2802%2900292-4"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1158%2F0008-5472.CAN-03-1501"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1159%2F000088512"}],"container-title":["Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/100878","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/100878","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:55:19Z","timestamp":1745463319000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000100878"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007]]},"references-count":18,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2007,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000100878","archive":["Portico"],"relation":{},"ISSN":["1018-8665","1421-9832"],"issn-type":[{"value":"1018-8665","type":"print"},{"value":"1421-9832","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007]]}}}