{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:31:27Z","timestamp":1791318687003,"version":"4.3.1"},"reference-count":22,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Vasc Res"],"published-print":{"date-parts":[[2007]]},"abstract":"<i>Background:<\/i> Shear stress induces coronary dilatation via production of nitric oxide (NO). This should involve the endothelial glycocalyx (EG). A greater effect was expected of albumin versus hydroxyethyl starch (HES) perfusion, because albumin seals coronary leaks more effectively than HES in an EG-dependent way. <i>Methods:<\/i> Isolated hearts (guinea pigs) were perfused at constant pressure with Krebs-Henseleit buffer augmented with 1\/3 volume 5% human albumin or 6% HES (200\/0.5 or 450\/0.7). Coronary flow was also determined after EG digestion (heparinase) and with nitro-<i>L<\/i>-arginine (NO-<i>L<\/i>-Ag). <i>Results:<\/i> Coronary flow (9.50 \u00b1 1.09, 5.10 \u00b1 0.49, 4.87 \u00b1 1.19 and 4.15 \u00b1 0.09 ml\/min\/g for \u2018albumin\u2019, \u2018HES 200\u2019, \u2018HES 450\u2019 and \u2018control\u2019, respectively, n = 5\u20136) did not correlate with perfusate viscosity (0.83, 1.02, 1.24 and 0.77 cP, respectively). NO-<i>L<\/i>-Ag and heparinase diminished dilatation by albumin, but not additively. Alone NO-<i>L<\/i>-Ag suppressed coronary flow during infusion of HES 450. Electron microscopy revealed a coronary EG of 300 nm, reduced to 20 nm after heparinase. Cultured endothelial cells possessed an EG of 20 nm to begin with. <i>Conclusions:<\/i> Albumin induces greater endothelial shear stress than HES, despite lower viscosity, provided the EG contains negative groups. HES 450 causes some NO-mediated dilatation via even a rudimentary EG. Cultured endothelial cells express only a rudimentary glycocalyx, limiting their usefulness as a model system.<\/jats:p>","DOI":"10.1159\/000104871","type":"journal-article","created":{"date-parts":[[2007,7,4]],"date-time":"2007-07-04T10:10:31Z","timestamp":1183543831000},"page":"435-443","source":"Crossref","is-referenced-by-count":80,"title":["The Endothelial Glycocalyx Prefers Albumin for Evoking Shear Stress-Induced, Nitric Oxide-Mediated Coronary Dilatation"],"prefix":"10.1159","volume":"44","author":[{"given":"Matthias","family":"Jacob","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Rehm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Loetsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joern O.","family":"Paul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Bruegger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Welsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Conzen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard F.","family":"Becker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2007,7,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1152%2Fajpheart.00297.2005"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097%2F00000542-200405000-00025"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1161%2F01.ATV.0000085630.24353.3D"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097%2F00000542-200606000-00018"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1161%2F01.RES.0000065917.53950.75"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159%2F000179238"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2796.2006.01625.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1113%2Fjphysiol.2003.058255"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1152%2Fajpheart.00218.2005"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cardiores.2006.11.021"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1161%2F01.RES.0000101744.47866.D5"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1161%2F01.ATV.0000225698.36212.6a"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS0008-6363%2898%2900032-7"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1097%2F00004647-200011000-00007"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2F0167-5699%2893%2990265-M"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2FS0962-8924%2800%2901897-3"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.270.44.26404"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.cb.08.110192.002053"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2796.2006.01620.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1152%2Fjapplphysiol.00740.2006"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1097%2F00003246-199704000-00008"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1152%2Fajpheart.00808.2003"}],"container-title":["Journal of Vascular Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/104871","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/104871","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T22:59:02Z","timestamp":1745449142000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000104871"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007]]},"references-count":22,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2007,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000104871","archive":["Portico"],"relation":{},"ISSN":["1018-1172","1423-0135"],"issn-type":[{"value":"1018-1172","type":"print"},{"value":"1423-0135","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007]]}}}