{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:19:55Z","timestamp":1790356795058,"version":"4.1.0"},"reference-count":24,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2007]]},"abstract":"<i>Background:<\/i> The 19-kD protein of buckwheat (BW) has been suggested to be a major allergen, but its characteristics and clinical significance are poorly defined. <i>Methods:<\/i> cDNA of the 19-kD BW allergen was cloned and expressed in <i>Escherichia coli<\/i>. Allergenicity and cross-allergenicity were confirmed by inhibition immunoblotting or by ELISA inhibition. The recombinant (r19-kD) protein was assessed for clinical utility in the diagnosis of BW reactivity in 18 BW-allergic and 19 BW-asymptomatic sensitized subjects using receiver operating characteristic analysis. <i>Results:<\/i> The 19-kD BW allergen, which is composed of 135 amino acids, has a weak homology to the vicilin-like allergens of cashew (<i>Ana<\/i> o 1), English walnut (<i>Jug r<\/i> 2) and 7 S globulin from <i>Sesamum indicum.<\/i> The r19-kD protein can inhibit sIgE binding to native 19-kD BW allergen. The maximum percentage inhibition of sIgE binding to crude BW extract was 56%. About 83.3% of the BW allergy patients had sIgE bound to r19-kD protein, compared to only 1 of the 19 BW-asymptomatic sensitized subjects. The areas under the receiver operating characteristic curves for the skin prick tests [0.925 (95% confidence interval: 0.839\u20131.012), p < 0.001] as well as r19-kD protein sIgE ELISAs [0.860 (95% confidence interval: 0.725\u20130.995), p <0.001] were higher than that of BW sIgE coated allergen particle test results [0.803 (95% confidence interval: 0.661\u20130.945), p = 0.002]. <i>Conclusions:<\/i> The 19-kD BW allergen may be the major allergen from BW. For the diagnosis of clinical reactivity to BW, the r19-kD protein sIgE ELISA test was more discriminative than the coated allergen particle sIgE measurement using whole BW extract.<\/jats:p>","DOI":"10.1159\/000106315","type":"journal-article","created":{"date-parts":[[2007,7,23]],"date-time":"2007-07-23T14:23:17Z","timestamp":1185200597000},"page":"267-274","source":"Crossref","is-referenced-by-count":29,"title":["Characterization of Buckwheat 19-kD Allergen and Its Application for Diagnosing Clinical Reactivity"],"prefix":"10.1159","volume":"144","author":[{"given":"Soo-Young","family":"Choi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung-Ho","family":"Sohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Won","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun-Kyung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chein-Soo","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung-Won","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2007,7,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1080%2F20014091091887"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jplph.2004.01.010"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1271%2Fbbb.68.1409"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1021%2Fjf0011485"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-5793%2896%2901367-1"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1398-9995.2000.00763.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1398-9995.2003.00412.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1159%2F000065173"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159%2F000024431"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1067%2Fmai.2002.125208"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2899%2970029-1"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1067%2Fmai.2001.114708"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2005.02150.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.2001.01113.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.2000.00928.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1067%2Fmai.2002.124775"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2897%2970220-3"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1398-9995.2000.00430.x"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.2001.01218.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2899%2970169-7"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1097%2F00130832-200406000-00017"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2899%2970171-5"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1159%2F000088720"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaci.2006.04.057"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/106315","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/106315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:37:34Z","timestamp":1745462254000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000106315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007]]},"references-count":24,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2007,11,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000106315","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007]]}}}