{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T09:19:49Z","timestamp":1776849589622,"version":"3.51.2"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[1997,10,22]],"date-time":"1997-10-22T00:00:00Z","timestamp":877478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[1997,10,22]],"date-time":"1997-10-22T00:00:00Z","timestamp":877478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dement Geriatr Cogn Disord"],"abstract":"<jats:p>Patients with probable late-onset Alzheimer''s disease (1-AD) had higher levels of serum \u03b1&lt;sub&gt;1&lt;\/sub&gt;-antichymotrypsin (\u03b1&lt;sub&gt;1&lt;\/sub&gt;-ACT) than those found in patients with vascular dementia (VD) and healthy elderly controls, when assessed by a competitive enzyme-linked immune assay. Serum \u03b1&lt;sub&gt;1&lt;\/sub&gt;-ACT was also characterized by SDS PAGE electrophoresis, Western blot and computer-assisted optical density reading (OD). Using a polyclonal affinity-purified antibody specific for human \u03b1&lt;sub&gt;1&lt;\/sub&gt;-ACT, one band with the apparent MW of 60 and another with 180 kD in sera from all subjects were clearly detectable. OD of both \u03b1&lt;sub&gt;1&lt;\/sub&gt;-ACT bands from patients with 1-AD was higher than that from VD patients, the 180-kD form being 2.65 times higher than that observed from patients with VD. Serum levels of other acute phase proteins from 1-AD were comparable to those observed in VD patients. A slight but nonstatistical increment of serum IL-6 was noted in patients with 1-AD. Serum \u03b1&lt;sub&gt;1&lt;\/sub&gt;-ACT was purified from 3 of these 1-AD patients by a two-step affinity chromatography technique. After Western blot, purified \u03b1&lt;sub&gt;1&lt;\/sub&gt;-ACT showed two or three different bands which immune-reacted with an antibody specific for \u03b1&lt;sub&gt;1&lt;\/sub&gt;-ACT. The apparent MWs were 60, 120 and 180 kD. In human sera the serpin was present mainly in a monomeric form, but it could also form SDS stable dimers and trimers. Both monomeric and SDS stable polymeric forms of \u03b1&lt;sub&gt;1&lt;\/sub&gt;-ACT appeared to be increased in sera from patients with 1-AD.<\/jats:p>","DOI":"10.1159\/000106652","type":"journal-article","created":{"date-parts":[[2007,7,25]],"date-time":"2007-07-25T05:36:15Z","timestamp":1185341775000},"page":"337-342","source":"Crossref","is-referenced-by-count":9,"title":["Monomeric and Polymeric Forms of Alpha-1 Antichymotrypsin in Sera from Patients with Probable Late Onset Alzheimer's Disease"],"prefix":"10.1159","author":[{"given":"Federico","family":"Licastro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Sirri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Trer\u00e8","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisabeth Jane","family":"Davis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1997,10,22]]},"container-title":["Dementia and Geriatric Cognitive Disorders"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/106652","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000106652","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T08:19:32Z","timestamp":1776845972000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000106652"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,10,22]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000106652","archive":["Portico"],"relation":{},"ISSN":["1420-8008","1421-9824"],"issn-type":[{"value":"1420-8008","type":"print"},{"value":"1421-9824","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,10,22]]}}}