{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:01:14Z","timestamp":1790913674195,"version":"4.1.0"},"reference-count":26,"publisher":"S. Karger AG","issue":"2-4","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytogenet Genome Res"],"published-print":{"date-parts":[[2007]]},"abstract":"Amplification of 8q is frequently found in gastroesophageal junction (GEJ) cancer. It is usually detected in high-grade, high-stage GEJ adenocarcinomas. Moreover, it has been implicated in tumor progression in other cancer types. In this study, a detailed genomic analysis of 8q was performed on a series of GEJ adenocarcinomas, including 22 primary adenocarcinomas, 13 cell lines and two xenografts, by array comparative genomic hybridization (aCGH) with a whole chromosome 8q contig array. Of the 37 specimens, 21 originated from the esophagus and 16 were derived from the gastric cardia. Commonly overrepresented regions were identified at distal 8q, i.e. 124\u2013125 Mb (8q24.13), at 127\u2013128 Mb (8q24.21), and at 141\u2013142 Mb (8q24.3). From these regions six genes were selected with putative relevance to cancer: <i>ANXA13, MTSS1, FAM84B <\/i>(alias <i>NSE2<\/i>)<i>, MYC, C8orf17 <\/i>(alias <i>MOST-1<\/i>) and <i>PTK2<\/i> (alias <i>FAK<\/i>). In addition, the gene <i>EXT1<\/i> was selected since it was found in a specific amplification in cell line SK-GT-5. Quantitative RT-PCR analysis of these seven genes was subsequently performed on a panel of 24 gastroesophageal samples, including 13 cell lines, two xenografts and nine normal stomach controls. Significant overexpression was found for <i>MYC<\/i> and <i>EXT1<\/i> in GEJ adenocarcinoma cell lines and xenografts compared to normal controls. Expression of the genes <i>MTSS1, FAM84B<\/i> and <i>C8orf17<\/i> was found to be significantly decreased in this set of cell lines and xenografts. We conclude that, firstly, there are other genes than <i>MYC<\/i> involved in the 8q amplification in GEJ cancer. Secondly, the differential expression of these genes contributes to unravel the biology of GEJ adenocarcinomas.<\/jats:p>","DOI":"10.1159\/000108293","type":"journal-article","created":{"date-parts":[[2007,11,14]],"date-time":"2007-11-14T10:29:10Z","timestamp":1195036150000},"page":"130-137","source":"Crossref","is-referenced-by-count":27,"title":["High-resolution array comparative genomic hybridization of chromosome 8q: evaluation of putative progression markers for gastroesophageal junction adenocarcinomas"],"prefix":"10.1159","volume":"118","author":[{"given":"M.","family":"van Duin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"van Marion","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.J.","family":"Vissers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.C.J.","family":"Hop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.N.M.","family":"Dinjens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.W.","family":"Tilanus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.D.","family":"Siersema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"van Dekken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2007,11,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M210184200"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038%2Fng1095-137"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002%2F1097-0142%2819930801%2972%3A3%3C649%3A%3AAID-CNCR2820720305%3E3.0.CO%3B2-L"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093%2Fije%2F29.4.645"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM198510033131404"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1207345"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007%2Fs004280000358"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1053%2Fgast.2001.27215"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002%2Fgcc.10294"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1101%2Fgr.210902"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.neo.7900231"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1136%2Fgut.33.11.1454"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291098-2264%28199808%2922%3A4%3C305%3A%3AAID-GCC6%3E3.0.CO%3B2-Z"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038%2Fnrc842"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038%2Fng754"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2894%2990349-2"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1067%2Fmsy.2003.244"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002%2Fgcc.1166"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1136%2Fmp.56.2.109"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1136%2Fgut.52.2.174"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1002%2Fgcc.20315"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1002%2Fgcc.20259"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1038%2Fmodpathol.3880143"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1207474"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1007%2Fs004390050415"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1042%2FBJ20021962"}],"container-title":["Cytogenetic and Genome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/108293","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/108293","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:57:33Z","timestamp":1745463453000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000108293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007]]},"references-count":26,"journal-issue":{"issue":"2-4","published-online":{"date-parts":[[2007,11,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000108293","archive":["Portico"],"relation":{},"ISSN":["1424-8581","1424-859X"],"issn-type":[{"value":"1424-8581","type":"print"},{"value":"1424-859X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007]]}}}