{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T04:13:06Z","timestamp":1776831186756,"version":"3.51.2"},"reference-count":50,"publisher":"S. Karger AG","issue":"2-4","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytogenet Genome Res"],"published-print":{"date-parts":[[2007]]},"abstract":"<jats:p>Rearrangements involving the &lt;i&gt;IGH&lt;\/i&gt; gene have been identified in about 50% of non-Hodgkin B-cell lymphomas (NHLs) and correlated to clinically relevant subgroups. However, the detection rate largely varied with the technique used. We analyzed the incidence of &lt;i&gt;IGH&lt;\/i&gt; rearrangements using several fluorescence in situ hybridization (FISH) techniques on metaphases obtained from 96 patients with nodal NHL. An &lt;i&gt;IGH&lt;\/i&gt; rearrangement was identified in 71 cases (74%). A t(14;18)(q32;q21) was found in 37 of the 42 follicular lymphomas (88.1%) studied and a t(11;14)(q13;q32) in 12 of the 14 mantle cell lymphomas (85.7%). &lt;i&gt;IGH&lt;\/i&gt; rearrangements were identified in 21 of the 40 diffuse large B-cell lymphomas (52.5%), including seven t(14;18)(q32;q21) and four t(3;14)(q27;q32). Conventional cytogenetics was uninformative in several cases. However, the complemented analysis using 24-color FISH, chromosomal whole paints, telomeric probes and locus specific identifiers enabled us to characterize complex and\/or masked &lt;i&gt;IGH&lt;\/i&gt; translocations in follicular lymphomas and mantle cell lymphomas and to identify all the chromosomal partners involved in &lt;i&gt;IGH&lt;\/i&gt; rearrangements in diffuse large B-cell lymphomas. This study shows the interest of using metaphase FISH in addition to conventional cytogenetics. Following banding techniques, FISH with the &lt;i&gt;IGH &lt;\/i&gt;dual color probe can be the first approach in NHL, after which chromosome painting and 24-color FISH can be used to identify the chromosomal partners involved in &lt;i&gt;IGH&lt;\/i&gt; rearrangements. The identification of these genes is of utmost importance for a better understanding of the molecular mechanisms involved in the genesis of lymphoma.<\/jats:p>","DOI":"10.1159\/000108319","type":"journal-article","created":{"date-parts":[[2007,11,14]],"date-time":"2007-11-14T10:29:10Z","timestamp":1195036150000},"page":"345-352","source":"Crossref","is-referenced-by-count":17,"title":["Molecular cytogenetics of IGH rearrangements in non-Hodgkin B-cell lymphoma"],"prefix":"10.1159","volume":"118","author":[{"given":"I.","family":"Bernicot","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Douet-Guilbert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.-J.","family":"Le 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