{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T19:30:23Z","timestamp":1776713423350,"version":"3.51.2"},"reference-count":31,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2007,9,20]],"date-time":"2007-09-20T00:00:00Z","timestamp":1190246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,9,20]],"date-time":"2007-09-20T00:00:00Z","timestamp":1190246400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Exp Nephrol"],"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease that is caused by a mutation of a single gene inherited from either parent. Mutations in the Pkd1 gene result in the formation of multiple fluid-filled cysts in kidneys. In previous studies, the functional regulatory sequences of Pkd1 promoter region were detected by the use of comparative genome analysis. &lt;i&gt;Methods:&lt;\/i&gt; To investigate the transcriptional regulation of the Pkd1 gene, the Pkd1 promoter was isolated. This promoter contains three Sp1-binding sites. Two of the sites which are found in a 300 bp fragment (\u2013127 to +157) were mutated. An electrophoretic mobility shift assay (EMSA) was performed to determine which transcription factors are bound to Pkd1. &lt;i&gt;Results:&lt;\/i&gt; Based on studies using a luciferase assay, the Sp1-A site (the nearest Sp1 to the ATG start codon) is more important for activation of Pkd1. The result of EMSA showed that Sp1 transcription factor binds with Pkd1 promoter regions. &lt;i&gt;Conclusions:&lt;\/i&gt; Two of the Sp1 sites were found in a proximal promoter region of Pkd1 (\u2013127 to +157). Sp1 sites affect an important role in the activation of the gene. Especially, the Sp1-A site is more important for expression of Pkd1.<\/jats:p>","DOI":"10.1159\/000108643","type":"journal-article","created":{"date-parts":[[2007,9,21]],"date-time":"2007-09-21T12:08:04Z","timestamp":1190376484000},"page":"e57-e64","source":"Crossref","is-referenced-by-count":3,"title":["Expression of the Pkd1 Gene Is Momentously Regulated by Sp1"],"prefix":"10.1159","volume":"107","author":[{"given":"Jeong Ok","family":"Jeon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Hyun","family":"Yoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong Hoon","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2007,9,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1093%2Fhmg%2F8.10.1861"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1006%2Fmgme.1999.2943"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291096-8628%2819980305%2976%3A2%3C137%3A%3AAID-AJMG6%3E3.0.CO%3B2-Q"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159%2F000106614"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM199307293290508"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM199507063330104"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1006%2Fgeno.2000.6197"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1440-1797.2006.00535.x"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2894%2990137-6"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1093%2Fhmg%2F4.4.575"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M201875200"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.272.11.7151"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1136%2Fjmg.39.7.463"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-1119%2899%2900357-1"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.270.43.25879"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.1998.9964"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS0300-483X%2802%2900577-2"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.ejhg.5201392"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1053%2Fsnep.2001.20929"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1097%2F01.ASN.0000028643.17901.42"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.1999.00370.x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2F0168-9525%2893%2990149-C"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.87.12.4509"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M203570200"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1128%2FMCB.21.14.4670-4683.2001"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1204038"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1204034"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1002%2Fjcb.20012"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.211191098"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1038%2F71724"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.7973635"}],"container-title":["Nephron Experimental Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/108643","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/108643","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:01:24Z","timestamp":1745463684000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000108643"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9,20]]},"references-count":31,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2007,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000108643","archive":["Portico"],"relation":{},"ISSN":["1660-2129"],"issn-type":[{"value":"1660-2129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9,20]]}}}