{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:01:58Z","timestamp":1790110918480,"version":"4.0.1"},"reference-count":23,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2007,10,19]],"date-time":"2007-10-19T00:00:00Z","timestamp":1192752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,10,19]],"date-time":"2007-10-19T00:00:00Z","timestamp":1192752000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Nephrol"],"published-print":{"date-parts":[[2008]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Increasing evidence suggests that connective tissue growth factor (CTGF) is involved in the epithelial-to-mesenchymal transition (EMT). The exact intracellular events that drive this process, however, are not fully understood. In this study, we investigated the role of integrin-linked kinase (ILK) in mediating CTGF-induced EMT. &lt;i&gt;Methods:&lt;\/i&gt; The expression of \u03b1-smooth muscle actin (\u03b1-SMA) and E-cadherin upon the stimulation by recombinant human CTGF (rhCTGF) in cultured human tubular epithelial cell line (HK-2) was detected by real-time RT-PCR and Western blot. Subsequently, the role of ILK was determined by using ILK siRNA. &lt;i&gt;Results:&lt;\/i&gt; rhCTGF increased the mRNA expression of \u03b1-SMA significantly in a dose- and time-dependent manner, while E-cadherin mRNA decreased in a dose- and time-dependent manner. \u03b1-SMA protein was up-regulated after stimulation by 5 ng\/ml CTGF for 96 h, and increased further after stimulation by 50 ng\/ml. An immunocytochemical study showed that \u03b1-SMA was initially detectable at 48 h, and increased further at 72 h, while there was almost no \u03b1-SMA immunostaining observed in the control group at the same time point. E-cadherin protein was also down-regulated in a dose-dependent manner. Transfection of HK-2 cells with ILK-siRNA significantly attenuated rhCTGF-induced \u03b1-SMA induction and E-cadherin repression. &lt;i&gt;Conclusion:&lt;\/i&gt; Our study suggested that ILK mediated the effect of EMT in proximal tubular epithelial cells stimulated by CTGF.<\/jats:p>","DOI":"10.1159\/000110019","type":"journal-article","created":{"date-parts":[[2007,10,19]],"date-time":"2007-10-19T11:00:58Z","timestamp":1192791658000},"page":"143-151","source":"Crossref","is-referenced-by-count":26,"title":["Inhibition of Integrin-Linked Kinase via a siRNA Expression Plasmid Attenuates Connective Tissue Growth Factor-Induced Human Proximal Tubular Epithelial Cells to Mesenchymal Transition"],"prefix":"10.1159","volume":"28","author":[{"given":"Bi-Cheng","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Xia","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Dong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Cong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Liang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aled O.","family":"Phillips","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2007,10,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.130.2.393"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI200215518"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.114.6.1285"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1096%2Ffj.03-0699com"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cellbi.2004.09.003"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cca.2006.05.029"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038%2F379091a0"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.273.1.528"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI200317913"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1053%2Fj.gastro.2005.06.067"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M008087200"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1101%2Fgad.880001"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1068999"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1101%2Fgad.862301"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cca.2007.03.029"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00109-003-0517-9"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1097%2F01.ASN.0000130565.69170.85"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1681%2FASN.2006050525"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cyto.2006.10.009"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2FS0002-9440%2810%2962533-3"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016%2FS0002-9440%2810%2963020-9"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.lfs.2005.08.037"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1093%2Fndt%2Fgfl243"}],"container-title":["American Journal of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/110019","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/110019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:00:30Z","timestamp":1745460030000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000110019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10,19]]},"references-count":23,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2007,11,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000110019","archive":["Portico"],"relation":{},"ISSN":["0250-8095","1421-9670"],"issn-type":[{"value":"0250-8095","type":"print"},{"value":"1421-9670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10,19]]}}}