{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T13:25:56Z","timestamp":1780579556948,"version":"3.54.1"},"reference-count":17,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathobiology"],"published-print":{"date-parts":[[2007]]},"abstract":"<jats:p>&lt;i&gt;Objective:&lt;\/i&gt; Previous studies provided evidence that many factors contribute to retinal angiogenesis, including inducible nitric oxide synthase (iNOS), hypoxia-inducible factor-1\u03b1 (HIF-1\u03b1) and vascular endothelial growth factor (VEGF). But the role of nitric oxide generated by iNOS in the regulation of expression of hypoxia-inducible genes in retinopathy of prematurity remains unclear. So we sought to better define the molecular basis of this iNOS-dependent regulation. &lt;i&gt;Methods:&lt;\/i&gt; In this study, using immunohistochemistry, real-time PCR and Western blotting technologies, we investigated the changes of iNOS, HIF-1\u03b1, VEGF and phosphatidylinositol 3-kinase\/Akt (PI3K\/Akt) expressions. &lt;i&gt;Results:&lt;\/i&gt; Hypoxia- induced overexpression of iNOS, HIF-1\u03b1, VEGF, PI3K\/Akt and phosphorylated PI3K\/Akt was observed in the untreated retinopathy of the prematurity group. Administration of the selective iNOS inhibitor aminoguanidine hemisulfate markedly decreased the expression of these genes. &lt;i&gt;Conclusions:&lt;\/i&gt; These results indicate that iNOS mediates HIF-1\u03b1 activation and VEGF expression in retinal angiogenesis and that the PI3K\/Akt signaling pathway may play a role in this process.<\/jats:p>","DOI":"10.1159\/000110027","type":"journal-article","created":{"date-parts":[[2007,12,14]],"date-time":"2007-12-14T10:31:44Z","timestamp":1197628304000},"page":"336-343","source":"Crossref","is-referenced-by-count":22,"title":["Inducible Nitric Oxide Synthase Mediates Hypoxia-Induced Hypoxia-Inducible Factor-1\u03b1 Activation and Vascular Endothelial Growth Factor Expression in Oxygen-Induced Retinopathy"],"prefix":"10.1159","volume":"74","author":[{"given":"Tao","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Ai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Hui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Qiao","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2007,12,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.freeradbiomed.2007.01.021"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.arcmed.2005.11.012"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038%2F359843a0"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1167%2Fiovs.05-0518"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.preteyeres.2007.01.004"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.plipres.2005.10.001"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.pathophys.2006.02.001"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.niox.2005.11.006"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093%2Femboj%2Fcdg117"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1167%2Fiovs.04-0052"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1139%2Fo05-047"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.bone.2004.02.012"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1042%2FBJ20031155"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.2000.3896"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.bcp.2006.06.038"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1158%2F1541-7786.MCR-05-0234"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.2006-1394"}],"container-title":["Pathobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/110027","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/110027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:00:37Z","timestamp":1745460037000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000110027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007]]},"references-count":17,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2007,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000110027","archive":["Portico"],"relation":{},"ISSN":["1015-2008","1423-0291"],"issn-type":[{"value":"1015-2008","type":"print"},{"value":"1423-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007]]}}}