{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T22:36:30Z","timestamp":1789425390497,"version":"build-2803163510"},"reference-count":31,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dermatology"],"published-print":{"date-parts":[[2008]]},"abstract":"<jats:p>&lt;i&gt;Objective:&lt;\/i&gt; We evaluated whether immunoblotting is capable of substantiating the posttreatment clinical assessment of patients with erythema migrans (EM), the hallmark of early Lyme borreliosis. &lt;i&gt;Methods:&lt;\/i&gt; In 50 patients, seroreactivity to different antigens of &lt;i&gt;Borrelia burgdorferi&lt;\/i&gt; sensu lato was analyzed by a recombinant immunoblot test (IB) in consecutive serum samples from a minimum follow-up period of 1 year. Antigens in the IgG test were decorin-binding protein A, internal fragment of p41 (p41i), outer surface protein C (OspC), p39, variable major protein-like sequence expressed (VlsE), p58 and p100; those in the IgM test were p41i, OspC and p39. Immune responses were correlated with clinical and treatment-related parameters. &lt;i&gt;Results:&lt;\/i&gt; Positive IB results were found in 50% before, in 57% directly after therapy and in 44% by the end of the follow-up for the IgG class, and in 36, 43 and 12% for the IgM class. In acute and convalescence phase sera, VlsE was most immunogenic on IgG testing (60 and 70%), and p41i (46 and 57%) and OspC (40 and 57%) for the IgM class. By the end of the follow-up, only the anti-p41i IgM response was significantly decreased to 24%. &lt;i&gt;Conclusions:&lt;\/i&gt; No correlation was found between IB results and treatment-related parameters. Thus, immunoblotting does not add to the clinical assessment of EM patients after treatment.<\/jats:p>","DOI":"10.1159\/000111505","type":"journal-article","created":{"date-parts":[[2008,1,28]],"date-time":"2008-01-28T07:48:52Z","timestamp":1201506532000},"page":"93-103","source":"Crossref","is-referenced-by-count":16,"title":["Immunoblot Analysis of the Seroreactivity to Recombinant &lt;i&gt;Borrelia burgdorferi &lt;\/i&gt;sensu lato Antigens, Including VlsE, in the Long-Term Course of Treated Patients with Erythema Migrans"],"prefix":"10.1159","volume":"216","author":[{"given":"Martin","family":"Glatz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Fingerle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina","family":"Wilske","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Ambros-Rudolph","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut","family":"Kerl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert R.","family":"M\u00fcllegger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2008,1,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM200107123450207"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159%2F000090657"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1001%2Farchderm.133.1.33"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01780663"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM200107123450202"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1136%2Fmp.50.4.186"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1054%2Fxd.2000.7179"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1001%2Farchderm.142.7.862"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.41.3.1299-1303.2003"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1128%2FCMR.18.3.484-509.2005"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1086%2F314053"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00579624"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00193630"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1007%2Fs004300050095"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007%2Fs004300050116"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2958.1998.01103.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS0092-8674%2800%2980206-8"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1128%2FIAI.68.4.2349-2352.2000"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1099%2F00221287-144-4-1033"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.43.8.3602-3609.2005"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1086%2F322669"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1080%2F07853890500431934"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ijmm.2006.09.001"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1007%2FBF02098084"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1086%2F374395"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1099%2Fjmm.0.05095-0"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1128%2FCVI.13.4.525-529.2006"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1086%2F323392"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1086%2F374376"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1016%2F0002-9343%2892%2990270-L"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1111%2F1523-1747.ep12462773"}],"container-title":["Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/111505","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/111505","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:17:03Z","timestamp":1745461023000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000111505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008]]},"references-count":31,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2008,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000111505","archive":["Portico"],"relation":{},"ISSN":["1018-8665","1421-9832"],"issn-type":[{"value":"1018-8665","type":"print"},{"value":"1421-9832","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008]]}}}