{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:45:07Z","timestamp":1759333507454,"version":"3.40.4"},"reference-count":6,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[2007]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Follicular lymphoma (FL) is one of the most common non-Hodgkin\u2019s lymphomas of B cells, being closely associated with a t(14;18) translocation. Detection of t(14;18), which is present in 70\u201395% of FL, might aid in FL diagnosis. &lt;i&gt;Objective:&lt;\/i&gt; To compare the efficacy of routine polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques in detecting t(14;18) in paraffin-embedded tissue samples of FL patients at different stages. Combined with other immunophenotypic biological determinants, detection of t(14;18) might help to determine patients at increased risk according to the FL International Prognostic Index (FLIPI) and therefore facilitate appropriate treatment. &lt;i&gt;Design and Methods:&lt;\/i&gt; This study was mainly based on a retrospective examination of formalin-fixed, paraffin-embedded lymph nodes. We selected fixed tissue samples of 21 FL patients treated at the National Cancer Institute Center in the period from 2000 to 2001. &lt;i&gt;Results:&lt;\/i&gt; FISH techniques could detect 14 of 18 FL cases with a sensitivity of 77.8%, while the PCR technique could detect only 11 of 18 FL cases with a sensitivity of 61.1%, resulting in a statistically significant difference between both techniques (p = 0.004). According to the FLIPI index, 9 of the 18 FL patients were categorized into the high-risk group (50%), 5 in the intermediated-risk group (27.8%) and 4 in the low-risk group (22.2%). &lt;i&gt;Conclusion:&lt;\/i&gt; The sensitivity of FISH is superior to that of PCR in the detection of the t(14;18) translocation in paraffin-embedded tissue samples. There is a statistically significant correlation between both CD10 and FISH with FLIPI.<\/jats:p>","DOI":"10.1159\/000112474","type":"journal-article","created":{"date-parts":[[2007,12,14]],"date-time":"2007-12-14T10:31:44Z","timestamp":1197628304000},"page":"231-236","source":"Crossref","is-referenced-by-count":5,"title":["Diagnostic and Prognostic Utility of t(14;18) in Follicular Lymphoma"],"prefix":"10.1159","volume":"118","author":[{"given":"H.","family":"Deghiedy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Fouda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Shahin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Shamaa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"El-Bedewy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Abd El-Ghaffar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2007,12,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.82.21.7439"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.leu.2403202"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1309%2FBLH8-MMK8-5UBQ-4K6R"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093%2Fannonc%2Fmdl162"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1093%2Fannonc%2Fmdi269"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1600-0609.2005.00564.x"}],"container-title":["Acta Haematologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/112474","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/112474","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:28:24Z","timestamp":1745461704000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000112474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007]]},"references-count":6,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2008,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000112474","archive":["Portico"],"relation":{},"ISSN":["0001-5792","1421-9662"],"issn-type":[{"type":"print","value":"0001-5792"},{"type":"electronic","value":"1421-9662"}],"subject":[],"published":{"date-parts":[[2007]]}}}