{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:45:10Z","timestamp":1761155110749,"version":"3.40.4"},"reference-count":24,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2007,12,17]],"date-time":"2007-12-17T00:00:00Z","timestamp":1197849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,12,17]],"date-time":"2007-12-17T00:00:00Z","timestamp":1197849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Exp Nephrol"],"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Stem cell factor (SCF) has been implicated in many disease processes characterized by tissue remodelling and fibrosis. The growth factor (SCF) was evaluated in a rat model of nephrotoxic serum nephritis (NTN), characterized by early inflammation followed by later tissue fibrosis&lt;i&gt;. &lt;\/i&gt;&lt;i&gt;Methods:&lt;\/i&gt; NTN was induced in male Wistar Kyoto rats using rabbit anti-rat glomerular basement membrane antibodies. Animals were sacrificed at days 7, 15, 30 and 45 (n = 4\u201310 per group). Rats\u2019 kidneys were immunostained for ED1 as marker of inflammation, CD34, SCF, c-kit, mast cell tryptase and markers of fibrosis; collagens III and IV and \u03b1-SMA. Changes in SCF protein and mRNA content were evaluated by Western blotting and Northern blotting, respectively. &lt;i&gt;Results:&lt;\/i&gt; In the NTN kidney, levels of immunoreactive SCF and SCF receptor (c-kit) were significantly higher in glomerular, tubular and interstitial compartments. Mast cells were barely detectable in NTN and control rat sections. Double immunostaining showed the co-localization of SCF with \u03b1-SMA and of the SCF receptor with CD34 and ED1 positive cells. Immunostainable SCF protein in each of the 3 compartments, glomerular, tubular and interstitial, showed a positive linear correlation with serum creatinine, proteinuria, glomerulosclerosis score and interstitial fibrosis scores. Using multivariate analysis, immunostainable tubular SCF was a predictor of glomerular sclerosis and immunostainable glomerular SCF predicted tubular atrophy. Increased SCF immunostain was not a consequence of altered transcription as there was a fall in SCF mRNA determined by Northern blotting. Western blotting of NTN kidney homogenates revealed two bands for SCF, a 43-kDa band which decreased, and a 19-kDa band which increased throughout the study. &lt;i&gt;Conclusion:&lt;\/i&gt; These results highlight the potential role of SCF and its receptor in the remodelling process of the NTN kidney. Upregulation of SCF may involve a translational mechanism, with the soluble SCF protein KL-S1 (19 kDa) being derived from the transmembrane SCF protein KL-1 (43 kD) by proteolytic cleavage. The immunohistochemical staining of few CD34+ cells in NTN kidneys warrants further evaluation of the nature of these cells in the context of the inflammatory as well as the fibrotic processes.<\/jats:p>","DOI":"10.1159\/000112518","type":"journal-article","created":{"date-parts":[[2007,12,19]],"date-time":"2007-12-19T07:33:29Z","timestamp":1198049609000},"page":"e1-e10","source":"Crossref","is-referenced-by-count":11,"title":["Stem Cell Factor in a Rat Model of Serum Nephrotoxic Nephritis"],"prefix":"10.1159","volume":"108","author":[{"given":"M.M.","family":"El Kossi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.L.","family":"Haylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.S.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.M.","family":"El Nahas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2007,12,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0168-8278%2897%2980113-4"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2003.00183.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2897%2970131-3"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291096-9896%28199912%29189%3A4%3C609%3A%3AAID-PATH474%3E3.0.CO%3B2-2"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2001.00783.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.1998.00159.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291521-4141%28199908%2929%3A08%3C2645%3A%3AAID-IMMU2645%3E3.3.CO%3B2-T"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0272-6386%2803%2900026-X"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1007%2Fs101570300005"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1136%2Fjcp.53.11.858"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038%2Fmodpathol.3880184"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1992.221"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00193492"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1159%2F000024283"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2F0165-0270%2894%2900184-I"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1053%2Fhupa.2001.28947"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jss.2004.01.005"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2890%2990301-T"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.94.17.9017"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.89.16.7350"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.174.1.125"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1097%2F00041552-200105000-00003"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2003.00255.x"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2FS0002-9440%2810%2963683-8"}],"container-title":["Nephron Experimental Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/112518","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/112518","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:28:49Z","timestamp":1745461729000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000112518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12,17]]},"references-count":24,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2008,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000112518","archive":["Portico"],"relation":{},"ISSN":["1660-2129"],"issn-type":[{"type":"electronic","value":"1660-2129"}],"subject":[],"published":{"date-parts":[[2007,12,17]]}}}