{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:25:37Z","timestamp":1758126337194,"version":"3.40.4"},"reference-count":26,"publisher":"S. Karger AG","issue":"5-6","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oncology"],"published-print":{"date-parts":[[2007]]},"abstract":"<i>Background:<\/i> Loss of heterozygosity (LOH) at 13q22 is a common event in nasopharyngeal carcinoma (NPC). <i>EDNRB<\/i> gene located at 13q22 has been demonstrated to be hypermethylated in some kinds of tumors. In the current study, we focused on the epigenetic and genetic alterations of <i>EDNRB<\/i> in NPC. <i>Methods:<\/i> The mRNA expression of <i>EDNRB<\/i> was detected by semiquantitative RT-PCR and real-time quantitative PCR in 49 NPC and 12 chronic nasopharyngitis biopsies. The methylation and LOH status of <i>EDNRB<\/i> were examined by methylation-specific polymerase chain reaction, microsatellite PCR and sequencing. We also examined the mRNA expression of <i>EDNRB<\/i> in four NPC cell lines after 5-aza-2\u2032-deoxycytidine treatment. <i>Results:<\/i><i>EDNRB<\/i> was downregulated in primary NPC tissues and NPC cell lines, and a relatively higher methylation level of <i>EDNRB<\/i> was found in NPC biopsies (84%) compared to that in chronic nasopharyngitis biopsies (42%). Treatment of NPC cell lines with 5-aza-2\u2032-deoxycytidine activated <i>EDNRB<\/i> expression. LOH of <i>EDNRB <\/i>gene was also found at two microsatellite sites with ratios of 6.25 and 16.67% in NPC. <i>Conclusion: <\/i>Our results suggested that <i>EDNRB<\/i> expression may be affected by aberrant promoter methylation and gene deletion and may play a role in the development of NPC.<\/jats:p>","DOI":"10.1159\/000113146","type":"journal-article","created":{"date-parts":[[2008,1,11]],"date-time":"2008-01-11T14:33:25Z","timestamp":1200062005000},"page":"357-363","source":"Crossref","is-referenced-by-count":18,"title":["Epigenetic and Genetic Alterations of the <i>EDNRB<\/i> Gene in Nasopharyngeal Carcinoma"],"prefix":"10.1159","volume":"72","author":[{"given":"Liang","family":"Zhou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangling","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjiao","family":"Shan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weidong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmei","family":"Yi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaitai","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caiping","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,1,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002%2Fgcc.20036"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1593%2Fneo.05217"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038%2F990040"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1006%2Fcbir.1998.0329"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2894%2990016-7"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1093%2Fhmg%2F6.10.1613"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1093%2Fhmg%2F10.9.903"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002%2Fijc.10271"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1745-7270.2006.00164.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1093%2Fnar%2F22.15.2990"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1207632"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1136%2Fjcp.56.1.52"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-1119%2899%2900014-1"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291098-2264%28199906%2925%3A2%3C169%3A%3AAID-GCC13%3E3.0.CO%3B2-I"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291097-0215%2819990812%2982%3A4%3C498%3A%3AAID-IJC5%3E3.0.CO%3B2-S"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1002%2F1098-2264%282000%299999%3A9999%3C%3A%3AAID-GCC1086%3E3.0.CO%3B2-D"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS1044579X02000883"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002%2Fijc.11166"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002%2Fgcc.20398"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1002%2Fijc.20593"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1209154"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1158%2F1078-0432.CCR-05-0267"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1002%2Fmc.20106"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1158%2F1078-0432.CCR-07-0085"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1093%2Fcarcin%2Fbgm116"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1158%2F1078-0432.CCR-04-0930"}],"container-title":["Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/113146","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/113146","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:36:29Z","timestamp":1745462189000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000113146"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007]]},"references-count":26,"journal-issue":{"issue":"5-6","published-online":{"date-parts":[[2008,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000113146","archive":["Portico"],"relation":{},"ISSN":["0030-2414","1423-0232"],"issn-type":[{"type":"print","value":"0030-2414"},{"type":"electronic","value":"1423-0232"}],"subject":[],"published":{"date-parts":[[2007]]}}}