{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T12:36:11Z","timestamp":1773318971696,"version":"3.50.1"},"reference-count":16,"publisher":"S. Karger AG","issue":"2-3","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb Physiol"],"published-print":{"date-parts":[[2008]]},"abstract":"<i>Mycobacterium austroafricanum <\/i>IFP 2012 is able to slowly grow on methyl <i>tert<\/i>-butyl ether (MTBE), a fuel oxygenate widely used as a gasoline additive. The potential of <i>M. austroafricanum<\/i> IFP 2012 for aerobic MTBE degradation was investigated in the presence of a secondary carbon source, isopropanol. The strain was then tested for MTBE biodegradation at the laboratory-scale in a fixed-bed reactor using perlite as the matrix, and isopropanol was injected once a week to maintain <i>M. austroafricanum<\/i> IFP 2012 biomass inside the perlite bed. The biofilter was operated for 85 days at an influent flow rate of 20 ml\/h by varying the MTBE concentration from 10 to 20 mg\/l. The hydraulic retention time was fixed at 5 days. The removal of MTBE depended on the inlet MTBE concentration and a MTBE removal efficiency higher than 99% was obtained for MTBE concentrations up to 15 mg\/l. A set of 16S rRNA gene primers specific for <i>M. austroafricanum<\/i> species was used to analyze the DNA extracted from the biofilter effluent in order to detect the presence of <i>M. austroafricanum<\/i> IFP 2012 and to estimate the effect of periodic injections of isopropanol on the release of the strain from the perlite bed. The results demonstrated that the injection of isopropanol served to maintain an active MTBE degrading biomass in the biofilter and that this system could be used to effectively treat MTBE contaminated groundwater.<\/jats:p>","DOI":"10.1159\/000121330","type":"journal-article","created":{"date-parts":[[2017,4,18]],"date-time":"2017-04-18T21:23:41Z","timestamp":1492550621000},"page":"190-198","source":"Crossref","is-referenced-by-count":10,"title":["Use of<i> Mycobacterium austroafricanum<\/i> IFP 2012 in a MTBE-Degrading Bioreactor"],"prefix":"10.1159","volume":"15","author":[{"given":"Helena","family":"Maciel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugues","family":"Mathis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Lopes Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darwin","family":"Lyew","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serge","family":"Guiot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Monot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles W.","family":"Greer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7oise","family":"Fayolle-Guichard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,7,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1021%2Fes001249j"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.2516%2Fogst%3A2003033"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1021%2Fes981337s"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1128%2FAEM.68.6.2754-2762.2002"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007%2Fs002530051423"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1021%2Fes003268z"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10295-003-0091-8"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00253-005-0074-y"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1897%2F04-641R.1"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1897%2F05-054R1.1"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2F0273-1223%2896%2900001-7"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0065-2164%2808%2970331-9"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1061%2F%28ASCE%290733-9372%282002%29128%3A9%28852%29"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1061%2F%28ASCE%290733-9372%282002%29128%3A9%28842%29"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10532-005-9034-6"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1021%2Fes030652y"}],"container-title":["Microbial Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/121330","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/121330","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:02:33Z","timestamp":1745460153000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000121330"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008]]},"references-count":16,"journal-issue":{"issue":"2-3","published-online":{"date-parts":[[2008,8,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000121330","archive":["Portico"],"relation":{},"ISSN":["2673-1665","2673-1673"],"issn-type":[{"value":"2673-1665","type":"print"},{"value":"2673-1673","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008]]}}}