{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T09:59:37Z","timestamp":1784023177657,"version":"3.55.0"},"reference-count":19,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2008]]},"abstract":"Although astragaloside IV, a saponin isolated from <i>Astragalus membranaceus<\/i>, has been shown to protect the myocardium against ischemia\/reperfusion injury, its effect on the status of sarcoplasmic reticulum (SR) Ca<sup>2+<\/sup> transport in the injured myocardium remains largely unknown. In this study, we investigated whether in cultured cardiomyocytes subjected to hypoxia and reoxygenation (H\/R) administration of astragaloside IV during H\/R attenuates the myocardial cell injury and prevents changes in Ca<sup>2+<\/sup> handling activities and gene expression of SR Ca<sup>2+<\/sup> pump. Cultured cardiomyocytes from neonatal rats were exposed to 6 h of hypoxia followed by 3 h of reoxygenation. Myocyte injury was determined by the release of cardiac troponin I in supernatant. Astragaloside IV significantly inhibited cardiac troponin I release after H\/R in a dose-dependent manner. The diastolic [Ca<sup>2+<\/sup>]<sub>i<\/sub> measured with Fura-2\/AM was significantly increased after reoxygenation. Astragaloside IV prevented the rise of diastolic [Ca<sup>2+<\/sup>]<sub>i<\/sub> and the depression of caffeine-induced Ca<sup>2+<\/sup> transients caused by H\/R. Furthermore, the observed depressions in SR Ca<sup>2+<\/sup>-ATPase activity as well as the mRNA and protein expression of SR Ca<sup>2+<\/sup>-ATPase in hypoxic-reoxygenated cardiomyocytes were attenuated by astragaloside IV treatment. These results suggest that the beneficial effect of astragaloside IV in H\/R-induced injury may be related to normalization of SR Ca<sup>2+<\/sup> pump expression and, thus, may prevent the depression in SR Ca<sup>2+<\/sup> handling.<\/jats:p>","DOI":"10.1159\/000121335","type":"journal-article","created":{"date-parts":[[2008,3,17]],"date-time":"2008-03-17T11:51:08Z","timestamp":1205754668000},"page":"325-332","source":"Crossref","is-referenced-by-count":57,"title":["Astragaloside IV Improved Intracellular Calcium Handling in Hypoxia-Reoxygenated Cardiomyocytes via the Sarcoplasmic Reticulum Ca<sup>2+<\/sup>-ATPase"],"prefix":"10.1159","volume":"81","author":[{"given":"Xiao-Le","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Jian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Yun","family":"Bian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Dan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Ping","family":"Bian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Nan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2008,3,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ejphar.2006.01.013"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2FS0163-7258%2800%2900103-0"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10863-005-9474-z"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1006%2Fjmcc.2001.1394"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1006%2Fjmcc.1998.0748"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2FS0008-6363%2803%2900429-2"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1152%2Fajpheart.01244.2003"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1055%2Fs-2005-873126"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cardiores.2004.11.019"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2FS0022-2828%2803%2900003-8"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1142%2FS0192415X06004028"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1097%2F00005344-199903000-00021"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS0197-4580%2803%2900035-6"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1142%2FS0192415X0600448X"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00797910"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1142%2FS0192415X05002618"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1258%2F002367706776319042"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1161%2Fhh1801.096264"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1023%2FA%3A1007120332587"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/121335","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/121335","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:02:34Z","timestamp":1745460154000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000121335"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008]]},"references-count":19,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2008,5,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000121335","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"value":"0031-7012","type":"print"},{"value":"1423-0313","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008]]}}}