{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T22:12:08Z","timestamp":1777759928094,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2008,3,18]],"date-time":"2008-03-18T00:00:00Z","timestamp":1205798400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2008,3,18]],"date-time":"2008-03-18T00:00:00Z","timestamp":1205798400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brain Behav Evol"],"abstract":"<jats:p>Each of 8 young adult female squirrel monkeys were injected via a femoral vein cannula with 167 \u00b5Ci\/100 g body weight of 2-(1,2\u20133H)-deoxy-D-glucose (2-DG) (New England Nuclear, 37,3 Ci\/mmol) in 0.5 ml sterile saline. 4 additional female squirrel monkeys were injected in the same manner with 100 \u00b5Ci\/100 g body weight of the (3H)-2-DG. 2 h after this initial injection the original 8 animals were injected with 16.7 \u00b5Ci\/100 g body weight of 2-(1-14C)-deoxy-D-glucose (51.3 mCi\/mmol) in 0.5 ml sterile saline. The 4 additional animals were injected with 25 \u00b5Ci\/100 g body weight of the (14C)-2-DG. Half of the animals at each dose level were restrained in the upright position with Velcro straps and a nontraumatic moulded plastic head holder on a modified animal restraint board [Withrow and Devine, 1972] with the head in the sagittal plane but tilted forward about 20\u00b0. They were then subjected to horizontal rotary motion at 25 rpm together with a vertical movement of 6 inches at 0.5 Hz for 1 h in a lighted room. The other half of the animals at each dose were restrained in the same manner and maintained in a quiescent state. At the end of this period each animal was anesthetized with ketamine, and the brain was quickly dissected out and frozen in isopentane cooled to \u201360\u00b0C with dry ice. Transverse cryostat sections (\u201315\u00b0C) of the brainstem were cut alternately at 200 and 20 \u00b5m from the nucleus gracilis caudally through the superior vestibular nucleus rostrally. Micropunch samples of the individual vestibular nuclei, and other brainstem nuclei and areas were obtained from the 200-\u00b5m sections with a modification of the method of Eik-Nes and Brizzee with a small stainless steel punch measuring 850 \u00b5m in diameter. The frozen punch samples were prepared for liquid scintillation counting (Beckman LS7500 system). Differential (3H) and (14C) counts (cpm) were made employing external standards. The 20-\u00b5m sections were prepared for 14C autoradiography by standard methods employing Kodak SB-5 X-ray film. The (14C) autoradiography from the motion-stimulated animals revealed a selectively high uptake of 2-DG in all of the vestibular nuclei, the lateral cuneate nucleus and the lateral reticular nucleus as compared with the corresponding structures in the control animals. The area postrema and nucleus of the solitary tract also exhibited somewhat higher grain density in motion-stimulated as compared with control subjects. The inferior olive showed a heavy 2-DG uptake in nearly all subjects but the brain density appeared somewhat higher in motion-stimulated animals. Data from the liquid scintillation counts revealed that the 14C\/3H ratio (cpm) was significantly higher (p &lt; 0.05) in the medial and inferior vestibular nuclei and nucleus cuneatus in motion-stimulated than in control animals. Values for the other vestibular nuclei and dorsal reticular formation (region of the vomiting center), and nucleus of the solitary tract were high but did not attain the level of statistical significance.<\/jats:p>","DOI":"10.1159\/000121483","type":"journal-article","created":{"date-parts":[[2008,3,19]],"date-time":"2008-03-19T09:02:41Z","timestamp":1205917361000},"page":"14-25","source":"Crossref","is-referenced-by-count":9,"title":["Motion-Induced Alterations in 2-Deoxyglucose Uptake in Brainstem Nuclei of Squirrel Monkeys: Autoradiographic and Liquid Scintillation Studies"],"prefix":"10.1159","author":[{"given":"K.R.","family":"Brizzee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.P.","family":"Dunlap","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,3,18]]},"container-title":["Brain, Behavior and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000121483","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:46:27Z","timestamp":1777470387000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000121483"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3,18]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000121483","archive":["Portico"],"relation":{},"ISSN":["0006-8977","1421-9743"],"issn-type":[{"value":"0006-8977","type":"print"},{"value":"1421-9743","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3,18]]}}}