{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T18:19:34Z","timestamp":1777918774969,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2008,3,25]],"date-time":"2008-03-25T00:00:00Z","timestamp":1206403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2008,3,25]],"date-time":"2008-03-25T00:00:00Z","timestamp":1206403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroendocrinology"],"abstract":"<jats:p>In vitro, median eminence was found to take up 3H-LRF rapidly, reach equilibrium in approximately 30 min, and achieve an uptake (30 pg LRF\/mg) twice that accumulated by anterior pituitary. Conditions which challenged cellular integrity (boiling, treatment with hypertonic saline, etc.) caused marked increases in uptake. Uptake was temperature-dependent and required oxygen and glucose, but was not noticeably ouabain-sensitive. The dissociation of 3H-LRF from median eminence occurred rapidly into media containing a 1,000-fold greater concentration of unlabeled LRF. Estradiol and testosterone, but not corticosterone, significantly inhibited uptake. Unlabeled LRF and TRF, but not MIF, provided significant competition for the uptake of 3H-LRF.<\/jats:p>","DOI":"10.1159\/000122303","type":"journal-article","created":{"date-parts":[[2008,3,25]],"date-time":"2008-03-25T11:33:59Z","timestamp":1206444839000},"page":"147-157","source":"Crossref","is-referenced-by-count":9,"title":["Transport Capacity of Median Eminence: in vitro Uptake of\n                    <sup>3<\/sup>\n                    H-LRF"],"prefix":"10.1159","author":[{"given":"Sharon S.","family":"Vaala","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.M.","family":"Knigge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,3,25]]},"container-title":["Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/122303","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000122303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T18:45:55Z","timestamp":1777661155000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000122303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3,25]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000122303","archive":["Portico"],"relation":{},"ISSN":["0028-3835","1423-0194"],"issn-type":[{"value":"0028-3835","type":"print"},{"value":"1423-0194","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3,25]]}}}