{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T18:33:48Z","timestamp":1777919628393,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2008,3,28]],"date-time":"2008-03-28T00:00:00Z","timestamp":1206662400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2008,3,28]],"date-time":"2008-03-28T00:00:00Z","timestamp":1206662400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroendocrinology"],"abstract":"<jats:p>Mediobasal hypothalamic (MBH) slices of adult ovariectomized (OVX) rats with or without 17\u03b2-estradiol (E&lt;sub&gt;2&lt;\/sub&gt;) pretreatment, were superfused in buffered (pH 7.2) oxygenated Locke medium containing bacitracin. Pulsatile or continuous administration of progesterone (10\u20137 or 10\u20138M) produced a marked increase in luteinizing hormone-releasing hormone (LHRH) release provided the animals had received E&lt;sub&gt;2&lt;\/sub&gt; prior to sacrifice. Omission of Ca++ in the medium, or addition of a Ca++ channel blocker (D-600, 10\u20134M), of a calmodulin inhibitor (trifluoperazine, 30 \u00b5M) or of a calmodulin-dependent tubulin kinase inhibitor (phenytoin, 50 \u00b5M), antagonized the stimulatory effect of progesterone. When sodium channels were blocked by tetrodotoxin (5 \u00d7 10\u20137M), the stimulatory effect of the steroid was completely abolished. The amplitude of the K+-induced LHRH release was slightly increased in the presence of progesterone (10\u20137M) but only from MBH slices of OVX-E&lt;sub&gt;2&lt;\/sub&gt;-treated rats. These results indicate that the secretory response of LHRH to progesterone requires priming with estradiol, is Ca++-dependent and involves mediation of calmodulin and a calmodulin-dependent kinase system.<\/jats:p>","DOI":"10.1159\/000124094","type":"journal-article","created":{"date-parts":[[2008,3,28]],"date-time":"2008-03-28T16:47:51Z","timestamp":1206722871000},"page":"325-331","source":"Crossref","is-referenced-by-count":22,"title":["Progesterone-Induced LHRH Release in vitro Is an Estrogen \u2013 as well as Ca\n                    <sup>++<\/sup>\n                    \u2013 and Calmodulin-Dependent Secretory Process"],"prefix":"10.1159","author":[{"given":"Sophia V.","family":"Drouva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliane","family":"Laplante","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Kordon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,3,28]]},"container-title":["Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/124094","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000124094","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T19:07:39Z","timestamp":1777662459000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000124094"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3,28]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000124094","archive":["Portico"],"relation":{},"ISSN":["0028-3835","1423-0194"],"issn-type":[{"value":"0028-3835","type":"print"},{"value":"1423-0194","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3,28]]}}}