{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T18:55:55Z","timestamp":1777920955341,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2008,4,9]],"date-time":"2008-04-09T00:00:00Z","timestamp":1207699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2008,4,9]],"date-time":"2008-04-09T00:00:00Z","timestamp":1207699200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroendocrinology"],"abstract":"<jats:p>Previously, we have demonstrated that dopamine (DA) stimulates growth hormone (GH) release from the goldfish pituitary through DA D1 receptors. In the present study, the role of cAMP in DA D1-stimulated GH release was investigated using static incubation of goldfish pituitary cells. The Dl agonist SKF38393 (1 nM\u201310 \u00b5M) induced GH release and cAMP accumulation in a dose-dependent manner with ED&lt;sub&gt;50s&lt;\/sub&gt; of 73 \u00b1 32 and 109 \u00b1 53 nM, respectively. In contrast, the D2 agonist LY171555 (1 nM\u201310 \u00b5M) was not effective in these regards. The GH-releasing action of SKF38393 was mimicked by the adenylate cyclase activator forskolin (0.1\u201340 \u00b5M) as well as the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (0.1 \u00b5M\u20131 mM). Dideoxyforskolin (0.1\u201340 \u00b5M), a derivative of forskolin inactive in stimulating adenylate cyclase, did not affect basal GH secretion. Similar stimulatory effects on GH release were also observed using the membrane-permeant cAMP analogs (10 \u00b5M\u20132 mM), dibutyryl cAMP and 8-bromo cAMP (8Br.cAMP). In the presence of a high dose (1 mM) of Br.cAMP, the ability of SKF38393 (1 nM-10 \u00b5M) to stimulate GH release was abolished, suggesting that the GH-releasing actions of cAMP and DA Dl stimulation are mediated through a common signal transduction mechanism. In the present study, the possible involvement of the cAMP-dependent enzyme protein kinase A (PKA) in DA Dl-stimulated GH release was also examined. The GH responses to 8Br.cAMP (1 mM) and SKF38393 (1 \u00b5M) were blocked by simultaneous treatment with the PKA inhibitor H89 (10 \u00b5M). The same dose (10 \u00b5M) of H89 did not affect the GH response to the protein kinase C activator sn-1,2-dioctanoyl glycerol (0.1 mM), indicating that this inhibitor is selective to PKA in the goldfish. Taken together, the present study provides evidence that DA Dl-stimulated GH release from goldfish pituitary cells is mediated through the adenylate cyclase-cAMP-PKA pathway.<\/jats:p>","DOI":"10.1159\/000126775","type":"journal-article","created":{"date-parts":[[2008,4,10]],"date-time":"2008-04-10T04:37:20Z","timestamp":1207802240000},"page":"410-417","source":"Crossref","is-referenced-by-count":16,"title":["Cyclic 3\u2019,5\u2019-Adenosine Monophosphate Mediates Dopamine D1-Stimulated Growth Hormone Release from Goldfish Pituitary Cells"],"prefix":"10.1159","author":[{"given":"Anderson On-Lam","family":"Wong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glen","family":"van der Kraak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John Philip","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,4,9]]},"container-title":["Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/126775","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000126775","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T19:40:47Z","timestamp":1777664447000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000126775"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,4,9]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000126775","archive":["Portico"],"relation":{},"ISSN":["0028-3835","1423-0194"],"issn-type":[{"value":"0028-3835","type":"print"},{"value":"1423-0194","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,4,9]]}}}