{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T18:55:55Z","timestamp":1777920955338,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2008,4,9]],"date-time":"2008-04-09T00:00:00Z","timestamp":1207699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2008,4,9]],"date-time":"2008-04-09T00:00:00Z","timestamp":1207699200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroendocrinology"],"abstract":"<jats:p>Meningioma benign tumors possess significant levels of 17\u03b2-hydroxysteroid dehydrogenase (17\u03b2-HSD) activity. Two different 17\u03b2-HSDs have been cloned and characterized. The cytosolic 17\u03b2-HSD I which exclusively catalyzes the interconversion of 17\u03b2-estradiol (E&lt;sub&gt;2&lt;\/sub&gt;) and estrone (E&lt;sub&gt;1&lt;\/sub&gt;) preferentially uses NADP+ and NADPH as cofactors. In contrast, the mitochondrial-microsomal 17\u03b2-HSD II catalyzes both the estrogenic as well as the androgenic substrates of the 17\u03b2-HSD and uses NAD+ and NADH as cofactors. We demonstrated here that the 17\u03b2-HSD activity in meningioma tissue homogenate is both estrogenic and androgenic with K&lt;sub&gt;m&lt;\/sub&gt; values of 2.4, 0.4, 14.7, and 2.0 \u00b5M for E&lt;sub&gt;2&lt;\/sub&gt;, E&lt;sub&gt;1&lt;\/sub&gt;, testosterone (T), and \u0394&lt;sub&gt;4&lt;\/sub&gt;-androstenedione (\u0394&lt;sub&gt;4&lt;\/sub&gt;), respectively. NAD+-NADH is almost exclusively used as cofactor in this tissue. Moreover, fractionation of meningioma tissue revealed that most of the 17\u03b2-HSD activity is present in the mitochondrial-microsomal fraction. Although Northern blot analysis on meningiomas with a specific probe for human 17\u03b2-HSD I showed no band, the specific cDNA probe of human 17\u03b2-HSD II hybridized at the expected size of 1.5 kb, which was also present in placenta. On four different meningioma tumors, we were able to correlate 17\u03b2-HSD II mRNA expression to high levels of 17\u03b2-HSD activity. Taken together, the present data suggest that the meningioma 17\u03b2-HSD could be the 17\u03b2-HSD II.<\/jats:p>","DOI":"10.1159\/000126779","type":"journal-article","created":{"date-parts":[[2008,4,10]],"date-time":"2008-04-10T04:37:20Z","timestamp":1207802240000},"page":"445-451","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of 17\u03b2-Hydroxysteroid Dehydrogenase Activity and mRNA Abundance in Human Meningioma Tumors"],"prefix":"10.1159","author":[{"given":"Jean-Louis","family":"Carsol","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre-Marie","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"de Launoit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,4,9]]},"container-title":["Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/126779","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000126779","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T19:40:46Z","timestamp":1777664446000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000126779"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,4,9]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000126779","archive":["Portico"],"relation":{},"ISSN":["0028-3835","1423-0194"],"issn-type":[{"value":"0028-3835","type":"print"},{"value":"1423-0194","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,4,9]]}}}