{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T17:46:57Z","timestamp":1773078417520,"version":"3.50.1"},"reference-count":26,"publisher":"S. Karger AG","issue":"3-4","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytogenet Genome Res"],"published-print":{"date-parts":[[2008]]},"abstract":"Idiopathic congenital clubfoot (CCF) is a type of congenital foot malformation, and previous studiesusing the extended transmission disequilibrium testing (ETDT) analysis have confirmed the <i>HOXD13<\/i> gene as the susceptible gene of CCF. This study aimed to verify whether Hoxd13 directly regulates skeletal muscle LIM protein 1 <i>(Fhl1)<\/i> expression during limb development in rat embryo, which will serve as a basis for comprehending etiological research of idiopathic CCF. Immunofluorescence staining was utilized to detect <i>Hoxd13<\/i> and <i>Fhl1<\/i> expression in 12.5d rat embryo, while luciferase assay, electrophoretic mobility shift assay (EMSA), and chromatin immunoprecipitation (ChIP) assay were used to confirm the interaction between the two genes. Both <i>Hoxd13<\/i> and <i>Fhl1<\/i> were expressed in the interdigital tissues of E12.5 rat embryo. Luciferase assay and EMSA identified a novel promoter region of <i>Fhl1<\/i> that directly interacts with Hoxd13. ChIP of the Hoxd13-<i>Fhl1 <\/i>promoter complex from the developing limb confirmed that endogenous Hoxd13 interacts with this region. Thus, Hoxd13 directly regulates <i>Fhl1 <\/i>expression in rat embryo. These findings suggest that HOXD13 may regulate the expression of <i>FHL1<\/i> in the development of idiopathic CCF.<\/jats:p>","DOI":"10.1159\/000138884","type":"journal-article","created":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T12:12:12Z","timestamp":1220271132000},"page":"189-195","source":"Crossref","is-referenced-by-count":21,"title":["HOXD13 may play a role in idiopathic congenital clubfoot by regulating the expression of <i>FHL1<\/i>"],"prefix":"10.1159","volume":"121","author":[{"given":"L.-L.","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.-N.","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Li-Ling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.-G.","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.-Y.","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.-L.","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,8,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1093%2Fhmg%2Fddh277"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1086%2F302041"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.crvi.2007.03.015"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1186%2F1471-2474-8-62"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1136%2Fjmg.37.9.680"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2F0888-7543%2891%2990482-T"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M707309200"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2F0925-4773%2891%2990067-G"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093%2Fmolehr%2F3.2.115"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002%2Fajmg.10776"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002%2Fbies.950190505"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-1119%2899%2900125-0"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1097%2F01.bpo.0000173248.96936.90"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1097%2F01.bpo.0000188994.90931.e8"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1136%2Fjmg.38.3.191"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2894%2990290-9"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-1119%2898%2900302-3"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1006%2Fmcbr.2000.0206"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1469-7580.2003.00147.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cellbi.2007.05.007"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.272.5261.548"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1097%2F01.bpb.0000111041.46580.b3"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1093%2Fnar%2F21.8.1687"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ydbio.2004.12.015"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1055%2Fs-2006-924372"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1097%2F00003086-197205000-00003"}],"container-title":["Cytogenetic and Genome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/138884","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/138884","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T01:20:47Z","timestamp":1745457647000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000138884"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008]]},"references-count":26,"journal-issue":{"issue":"3-4","published-online":{"date-parts":[[2008,8,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000138884","archive":["Portico"],"relation":{},"ISSN":["1424-8581","1424-859X"],"issn-type":[{"value":"1424-8581","type":"print"},{"value":"1424-859X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008]]}}}