{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:40:42Z","timestamp":1760121642309,"version":"3.40.4"},"reference-count":26,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intervirology"],"published-print":{"date-parts":[[2008]]},"abstract":"<i>Objective:<\/i> Activity of integrin\/ligand signaling leading to activation of small GTPases might regulate the efficiency of cell-to-cell spread of human T lymphotropic virus type I (HTLV-I) through the virological synapse. We compared both activity of small GTPases and involvement of integrin\/ligand signaling in extracellular release of HTLV-I virions between each three HTLV-I-infected T cell lines derived from HTLV-I-associated myelopathy\/tropical spastic paraparesis (HAM\/TSP) patients and from other origins as a control. <i>Methods:<\/i> Activity of small GTPases (Rho, Rac and Cdc42) was analyzed by pull-down assay with suppressive effect of both HTLV-I production and HTLV-I tax mRNA expression by anti-integrin-blocking antibodies. <i>Results:<\/i> All small GTPases were strongly activated in all cell lines derived from HAM\/TSP patients, but not in control cell lines except one cell line. Treatment of all cell lines derived from HAM\/TSP patients, but not all control cell lines, with anti-integrin-blocking antibodies significantly suppressed the level of HTLV-I p19 antigen in culture supernatants without downregulation of HTLV-I tax mRNA expression. <i>Conclusion:<\/i> Significant involvement with integrin\/ligand signaling in extracellular release of HTLV-I virions in cell lines derived from HAM\/TSP patients suggests that HTLV-I-infected cells in HAM\/TSP patients have the potential for the efficient spread of HTLV-I to uninfected cells.<\/jats:p>","DOI":"10.1159\/000139130","type":"journal-article","created":{"date-parts":[[2008,6,16]],"date-time":"2008-06-16T13:29:55Z","timestamp":1213622995000},"page":"123-129","source":"Crossref","is-referenced-by-count":12,"title":["HTLV-I Production Based on Activation of Integrin\/Ligand Signaling in HTLV-I-Infected T Cell Lines Derived from HAM\/TSP Patients"],"prefix":"10.1159","volume":"51","author":[{"given":"Naomi","family":"Fukushima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsufumi","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Nishiura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Ida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiyuki","family":"Aramaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsumi","family":"Eguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,6,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1423-0410.1984.tb00083.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1099%2Fvir.0.19334-0"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1080115"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2FS0955-0674%2803%2900109-1"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1097%2F01.moh.0000147892.83713.a7"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.C000635200"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0960-9822%2801%2900475-4"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0092-8674%2801%2900471-8"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2886%2991298-5"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2F0022-510X%2890%2990060-Z"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2F0306-9877%2893%2990111-3"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1080%2F13550280260422668"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2F0022-510X%2894%2990299-2"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jneuroim.2004.10.007"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002%2Fijc.2910340213"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.6980467"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.80.17.5402"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002%2Fijc.2910520316"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1620%2Ftjem.204.289"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M502639200"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2567.2005.02214.x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.3109%2F13550289809114225"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.96.7.3848"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1201906"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1182%2Fblood.V99.1.88"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1186%2F1742-4690-2-75"}],"container-title":["Intervirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/139130","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/139130","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T01:23:22Z","timestamp":1745457802000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000139130"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008]]},"references-count":26,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2008,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000139130","archive":["Portico"],"relation":{},"ISSN":["0300-5526","1423-0100"],"issn-type":[{"type":"print","value":"0300-5526"},{"type":"electronic","value":"1423-0100"}],"subject":[],"published":{"date-parts":[[2008]]}}}