{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:54:59Z","timestamp":1745470499019,"version":"3.40.4"},"reference-count":0,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[1976,1,1]],"date-time":"1976-01-01T00:00:00Z","timestamp":189302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1976,1,1]],"date-time":"1976-01-01T00:00:00Z","timestamp":189302400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells Tissues Organs"],"published-print":{"date-parts":[[1976]]},"abstract":"<jats:p>Gomori\u2019s metal precipitate technique was used to demonstrate the phosphatase activity of the human cervical lymph node in health and disease, using four different phosphate esters (sodium &lt;i&gt;\u03b2&lt;\/i&gt;-glycerophosphate and adenosine triphosphate at pH 9, riboflavin 5\u2019-phosphate at pH 9.2 and 5\u2019-monophosphoric acid at pH 8.3). In fetal lymph nodes, using 5\u2019-monophosphoric acid, an outstanding positive activity was noticed in the lymphatic follicles. With the other three substrates there was either no nodular reaction or just a narrow rim of positive activity around the follicles, the inter-nodular tissue being negative with all four substrates used. With chronic non-specific lymphadenitis the enzyme hydrolysing the three substrates (&lt;i&gt;\u03b2&lt;\/i&gt;-glycerophosphate, riboflavin 5\u2019-phosphate and adenosine triphosphate) began to make their appearance. It seems that with lymphadenitis, a &lt;i&gt;qualitative &lt;\/i&gt;change of the phosphatase activity takes place. A special characteristic pattern of phosphatase activity has been described in both \u2018early\u2019 and \u2018caseating\u2019 tuberculous lymphadenitis. In malignant lymphomas it was noticed that no activity was encountered with any of the four substrates in reticulum cell sarcoma. However, in lymphosarcoma a positive activity was obtained when either &lt;i&gt;\u03b2&lt;\/i&gt;-glycerophosphate or adenosine triphosphate substrates was used, to the extent that one can depend upon this characteristic phosphatase activity in differentiating between reticulum cell sarcoma and lymphosarcoma. However, no enzymatic activity was obtained when the other two phosphate esters were used.<\/jats:p>","DOI":"10.1159\/000144619","type":"journal-article","created":{"date-parts":[[2009,2,27]],"date-time":"2009-02-27T12:22:21Z","timestamp":1235737341000},"page":"279-286","source":"Crossref","is-referenced-by-count":0,"title":["Histochemistry of normal and diseased human lymph nodes"],"prefix":"10.1159","volume":"95","author":[{"given":"M.T.","family":"Rakhawy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.A.","family":"Tarkhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.M.","family":"Zakaria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,7,9]]},"container-title":["Cells Tissues Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/144619","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/144619","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T01:35:07Z","timestamp":1745458507000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000144619"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2004,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000144619","archive":["Portico"],"relation":{},"ISSN":["1422-6405","1422-6421"],"issn-type":[{"type":"print","value":"1422-6405"},{"type":"electronic","value":"1422-6421"}],"subject":[],"published":{"date-parts":[[1976]]}}}