{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:07:33Z","timestamp":1790842053549,"version":"4.1.0"},"reference-count":11,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2007,7,30]],"date-time":"2007-07-30T00:00:00Z","timestamp":1185753600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2007,7,30]],"date-time":"2007-07-30T00:00:00Z","timestamp":1185753600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemotherapy"],"published-print":{"date-parts":[[2008]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Prevalence of fluoroquinolone-resistant &lt;i&gt;Klebsiella oxytoca&lt;\/i&gt; has been reported worldwide. &lt;i&gt;Methods:&lt;\/i&gt; We recovered ten clinical &lt;i&gt;K. oxytoca&lt;\/i&gt; isolates from patients with acute cystitis, asymptomatic bacteriuria or acute bacillary diarrhea in Japan. Out of ten isolates, one fluoroquinolone-susceptible isolate was included as a control. Fluoroquinolone resistance was characterized genetically by PCR and DNA sequencing methods. Outer membrane protein (OMP) profiles were determined by SDS-PAGE. &lt;i&gt;Results:&lt;\/i&gt; In nine clinical isolates of levofloxacin-resistant &lt;i&gt;K. oxytoca&lt;\/i&gt;, nucleotide sequences in the quinolone-resistance-determining regions showed amino acid mutations such as Thr83Ile and Asp87Gly in GyrA and Ser80Ile in ParC. Combined effects of reduced 36-kDa OMP production and amino acid mutations in GyrA and ParC were shown by two &lt;i&gt;K. oxytoca&lt;\/i&gt; isolates exhibiting higher minimum inhibitory concentrations for fluoroquinolones than other fluoroquinolone-resistant isolates. &lt;i&gt;Conclusions:&lt;\/i&gt; In clinical &lt;i&gt;K. oxytoca&lt;\/i&gt; isolates, the various mechanisms of fluoroquinolone resistance may include reduced 36-kDa OMP production as well as GyrA and ParC mutations.<\/jats:p>","DOI":"10.1159\/000151266","type":"journal-article","created":{"date-parts":[[2008,8,14]],"date-time":"2008-08-14T09:41:55Z","timestamp":1218706915000},"page":"323-327","source":"Crossref","is-referenced-by-count":6,"title":["Fluoroquinolone Resistance in Clinical Isolates of &lt;i&gt;Klebsiella oxytoca&lt;\/i&gt;"],"prefix":"10.1159","volume":"54","author":[{"given":"Toshinobu","family":"Horii","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Osaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Muramatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2007,7,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2800%2902509-5"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1086%2F502313"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007%2Fs100960050014"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.2174%2F1568005043340623"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.46.12.3984-3986.2002"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1089%2F107662903322286472"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.47.10.3332-3335.2003"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1099%2F13500872-145-3-673"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159%2F000085612"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159%2F000098427"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1089%2F107662903765826831"}],"container-title":["Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/151266","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/151266","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T19:35:45Z","timestamp":1726169745000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/CHE\/article\/doi\/10.1159\/000151266"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7,30]]},"references-count":11,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2008,8,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000151266","archive":["Portico"],"relation":{},"ISSN":["0009-3157","1421-9794"],"issn-type":[{"value":"0009-3157","type":"print"},{"value":"1421-9794","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7,30]]}}}