{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:32:58Z","timestamp":1760707978963},"reference-count":5,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Urol Int"],"published-print":{"date-parts":[[2008]]},"abstract":"<jats:p>&lt;i&gt;Introduction:&lt;\/i&gt; This study investigates the feasibility of replacing urinary epithelial cells with oral keratinocytes seeded on a bladder acellular matrix graft (BAMG) to reconstruct a tissue-engineered urethra. &lt;i&gt;Materials and Methods:&lt;\/i&gt; After enzymatic treatment of a small segment of rabbit buccal mucosa (1.0 \u00d7 0.4 cm), the epidermis and dermis were separated mechanically. Oral keratinocytes were isolated from the epidermis and seeded onto a culture dish with a feeder layer of 3T3 mouse fibroblasts inhibited by mitomycin (i3T3) or on a culture dish without i3T3, respectively. These cells were expanded and applied to sterilized BAMG to obtain a tissue-engineered mucosa. Oral keratinocytes were assessed by growth curve, morphology and immunofluorescence staining. The tissue-engineered mucosa was assessed using morphology, immunohistochemistry staining and scanning electron microscopy. &lt;i&gt;Results:&lt;\/i&gt; Oral keratinocytes seeded onto a feeder layer of i3T3 had finer morphous and better amplification capability, and could be passaged for 8 generations. Oral keratinocytes that were cultured without i3T3 could only be subcultured 2 generations before ageing. Passage 2 oral keratinocytes cultured with i3T3 were expanded and seeded onto sterilized BAMG to obtain a tissue-engineered mucosa for urethral reconstruction. The compound graft was assessed using HE staining and scanning electron microscopy. Oral keratinocytes had good compatibility with BAMG. &lt;i&gt;Conclusions:&lt;\/i&gt; Rabbit oral keratinocytes of can be cultured in vitro and attain magnitude in quantity. Oral keratinocytes also had good compatibility with BAMG and the compound graft achieved could become a new choice in urethral reconstruction.<\/jats:p>","DOI":"10.1159\/000151406","type":"journal-article","created":{"date-parts":[[2008,10,17]],"date-time":"2008-10-17T07:47:16Z","timestamp":1224229636000},"page":"290-295","source":"Crossref","is-referenced-by-count":18,"title":["Preliminary Experimental Study of Tissue-Engineered Urethral Reconstruction Using Oral Keratinocytes Seeded on BAMG"],"prefix":"10.1159","volume":"81","author":[{"given":"Chao","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuemin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lujie","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuo","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,10,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.eururo.2006.11.039"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.urology.2005.10.033"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2FS0022-5347%2805%2966447-6"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2875%2990183-X"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1159%2F000096930"}],"container-title":["Urologia Internationalis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/151406","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/151406","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T19:40:47Z","timestamp":1715974847000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/UIN\/article\/doi\/10.1159\/000151406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008]]},"references-count":5,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2008,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000151406","archive":["Portico"],"relation":{},"ISSN":["0042-1138","1423-0399"],"issn-type":[{"value":"0042-1138","type":"print"},{"value":"1423-0399","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008]]}}}