{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:35:54Z","timestamp":1773300954647,"version":"3.50.1"},"reference-count":26,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2008,8,21]],"date-time":"2008-08-21T00:00:00Z","timestamp":1219276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,8,21]],"date-time":"2008-08-21T00:00:00Z","timestamp":1219276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2009]]},"abstract":"<i>Background:<\/i> Superoxide produced by airway epithelial and inflammatory cells may contribute to the pathogenesis of asthma. NADPH oxidase (an enzyme complex made up of 5 subunits) is a major source of superoxide in cells. We sought to elucidate the role of genetic variability in the gene encoding the p22<sup>phox<\/sup> subunit of the NADPH oxidase (CYBA, 16q24.3) in asthma susceptibility by means of an association study of haplotypes based on 3 common single nucleotide polymorphisms (SNPs), \u2013930A\/G, 242C\/T (H72Y) and 640A\/G. <i>Methods:<\/i> SNPs were genotyped by PCR-RFLP methods in a cohort of age- and sex-matched subjects with bronchial asthma (n = 305) and healthy controls (n = 311). Haplotypes were constructed in silico using a Monte Carlo-based algorithm. Furthermore, association of SNPs with sensitization to selected allergens (total 15 tested by skin prick test positivity\/negativity) was analyzed. <i>Results:<\/i> In a single locus analysis, SNPs 242C\/T and 640A\/G were marginally significantly associated with asthma (p = 0.036 and 0.036, respectively). SNP 640A\/G showed a significant association with sensitization to 2 allergens tested (p<sub>corr<\/sub> < 0.02). Haplotype analysis identified a total of 8 haplotypes with population frequencies from 0.07 to 0.22. Distribution of haplotypes significantly differed between cases and controls (omnibus p = 0.017, 10,000 permutations). In the post-hoc analysis, haplotype CYBA<sub>3<\/sub> (\u2013930G\/242T\/640A) was associated with an increased risk of asthma (p<sub>corr<\/sub> < 0.05; OR = 1.43, 95% CI 1.06\u20131.93). <i>Conclusions:<\/i> This study supports the hypothesis that genetic variability in the <i>CYBA<\/i> gene probably contributes to the susceptibility to bronchial asthma (or its related phenotypes) in our population.<\/jats:p>","DOI":"10.1159\/000151508","type":"journal-article","created":{"date-parts":[[2008,8,22]],"date-time":"2008-08-22T13:58:05Z","timestamp":1219413485000},"page":"73-80","source":"Crossref","is-referenced-by-count":16,"title":["Haplotype Analysis of the NADPH Oxidase p22<sup>phox<\/sup> Gene in Patients with Bronchial Asthma"],"prefix":"10.1159","volume":"148","author":[{"given":"Lydie","family":"Izakovicova Holla","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate\u0159ina","family":"Ka\u0148kov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Znojil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,8,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ejphar.2005.12.087"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.2174%2F1568010054526322"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.2174%2F1568010054526368"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097%2F01.mcp.0000198067.50457.71"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1179%2F135100005X70233"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.arcmed.2005.02.006"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaci.2004.05.013"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.pharmthera.2005.10.015"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.271.29.17404"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-5793%2803%2900331-4"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI114898"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1161%2F01.HYP.0000126579.50711.62"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1067%2Fmai.2001.118891"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1600-051X.2002.290409.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-2999%2801%2901320-6"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1186%2F1477-5751-5-5"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.atherosclerosis.2004.01.043"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1468-1331.2007.01904.x"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1080%2F10715760701796918"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2006.02458.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1136%2Fthx.2006.057935"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2006.02612.x"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1159%2F000094536"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2005.02342.x"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1517%2F13543784.14.9.1145"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1136%2Fthx.2004.022616"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/151508","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/151508","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T21:29:24Z","timestamp":1726176564000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/IAA\/article\/doi\/10.1159\/000151508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,8,21]]},"references-count":26,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2008,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000151508","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,8,21]]}}}