{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T01:10:02Z","timestamp":1745457002725,"version":"3.40.4"},"reference-count":23,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2008,9,6]],"date-time":"2008-09-06T00:00:00Z","timestamp":1220659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,9,6]],"date-time":"2008-09-06T00:00:00Z","timestamp":1220659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Genetic and developmental disruption of surfactant protein B (SP-B) expression causes neonatal respiratory distress syndrome (RDS). &lt;i&gt;Objectives:&lt;\/i&gt; To assess developmental and genetic regulation of SP-B expression in vivo. &lt;i&gt;Methods:&lt;\/i&gt; To evaluate in vivo developmental regulation of SP-B, we used immunoblotting to compare frequency of detection of mature and pro-SP-B peptides in developmentally distinct cohorts: 24 amniotic fluid samples, unfractionated tracheal aspirates from 101 infants \u226534 weeks\u2019 gestation with (75) and without (26) neonatal RDS, and 6 nonsmoking adults. To examine genetic regulation, we used univariate and logistic regression analyses to detect associations between common SP-B &lt;i&gt;(SFTPB)&lt;\/i&gt; genotypes and SP-B peptides in the neonatal RDS cohort. &lt;i&gt;Results:&lt;\/i&gt; We found pro-SP-B peptides in 24\/24 amniotic fluid samples and in 100\/101 tracheal aspirates from newborn infants but none in bronchoalveolar lavage from normal adults (0\/6) (p &lt; 0.001). We detected an association (p = 0.0011) between pro-SP-B peptides (M&lt;sub&gt;r&lt;\/sub&gt; 40 and 42 kDa) and genotype of a nonsynonymous single nucleotide polymorphism at genomic position 1580 that regulates amino-terminus glycosylation. &lt;i&gt;Conclusions:&lt;\/i&gt; Pro-SP-B peptides are more common in developmentally less mature humans. Association of genotype at genomic position 1580 with pro-SP-B peptides (M&lt;sub&gt;r&lt;\/sub&gt; 40 and 42 kDa) suggests genetic regulation of amino terminus glycosylation in vivo.<\/jats:p>","DOI":"10.1159\/000153095","type":"journal-article","created":{"date-parts":[[2008,9,7]],"date-time":"2008-09-07T19:40:56Z","timestamp":1220816456000},"page":"117-124","source":"Crossref","is-referenced-by-count":17,"title":["Developmental and Genetic Regulation of Human Surfactant Protein B in vivo"],"prefix":"10.1159","volume":"95","author":[{"given":"Aaron","family":"Hamvas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hillary B.","family":"Heins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan H.","family":"Guttentag","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel J.","family":"Wegner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle A.","family":"Trusgnich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate W.","family":"Bennet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher S.","family":"Carlson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. Sessions","family":"Cole","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,9,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1444-0903.2001.00200.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1080%2F08035250600615168"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00390-007-0809-3"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM199302113280606"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.jp.7211242"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.physiol.63.1.555"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1165%2Frcmb.2003-0262OC"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M312029200"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159%2F000292439"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1442-200X.2002.01605.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1186%2F1465-9921-6-80"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.271.33.19689"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.272.15.9640"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.275.12.8672"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI117173"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1042%2FBJ20021376"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1164%2Frccm.200304-479OC"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1203%2F01.PDR.0000145565.45490.D9"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1203%2FPDR.0b013e3180a03232"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1002%2Fhumu.9378"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M306844200"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1152%2Fajplung.00310.2003"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1165%2Frcmb.2002-0111OC"}],"container-title":["Neonatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/153095","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/153095","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:44:57Z","timestamp":1745455497000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000153095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9,6]]},"references-count":23,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2009,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000153095","archive":["Portico"],"relation":{},"ISSN":["1661-7800","1661-7819"],"issn-type":[{"type":"print","value":"1661-7800"},{"type":"electronic","value":"1661-7819"}],"subject":[],"published":{"date-parts":[[2008,9,6]]}}}