{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:18:21Z","timestamp":1790723901229,"version":"4.1.0"},"reference-count":10,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2008,10,24]],"date-time":"2008-10-24T00:00:00Z","timestamp":1224806400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,10,24]],"date-time":"2008-10-24T00:00:00Z","timestamp":1224806400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lifestyle Genomics"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p>&lt;i&gt;Background\/Aims:&lt;\/i&gt; Vitamin B&lt;sub&gt;12&lt;\/sub&gt; (B-12) is an essential cofactor for methionine synthase, and methionine is critical for the methylation of various biological molecules including DNA. Whether changes in B-12 levels can alter specific gene expression through DNA methylation and whether dietary methionine has any effect on general DNA methylation status still remains controversial. &lt;i&gt;Methods:&lt;\/i&gt; We raised severely B-12-deficient rats as well severely-B-12 deficient rats but supplemented with 5% methionine. mRNA levels of methionine cycle-related enzymes were analyzed. &lt;i&gt;Results:&lt;\/i&gt; Gene expression patterns changed under B-12-deficient conditions but were recovered by dietary methionine supplementation to B-12-deficient rats. However, cystathionine \u03b2-synthase mRNA levels, which had decreased under B-12-deficient conditions, did not recover with supplementary dietary methionine. The CpG island of the cystathionine \u03b2-synthase promoter was hypomethylated in B-12-deficient rats, and showed no recovery after methionine addition. &lt;i&gt;Conclusions:&lt;\/i&gt; Dietary B-12 can affect epigenetic machinery by regulating DNA methylation status and dietary methionine may have small effects on DNA methylation.<\/jats:p>","DOI":"10.1159\/000165374","type":"journal-article","created":{"date-parts":[[2008,10,24]],"date-time":"2008-10-24T08:25:14Z","timestamp":1224836714000},"page":"29-36","source":"Crossref","is-referenced-by-count":16,"title":["Change of Epigenetic Control of Cystathionine Beta-Synthase Gene Expression through Dietary Vitamin B&lt;sub&gt;12&lt;\/sub&gt; Is Not Recovered by Methionine Supplementation"],"prefix":"10.1159","volume":"2","author":[{"given":"Atsushi","family":"Uekawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keishuke","family":"Katsushima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asumi","family":"Ogata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsunori","family":"Kawata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nozomi","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken-Ichi","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Maekawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadahiro","family":"Tadokoro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2008,10,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.74.3.916"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.74.3.921"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2F0006-2952%2879%2990642-7"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093%2Fcarcin%2F18.5.869"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.3177%2Fjnsv.53.95"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.1993.1750"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1093%2Fnar%2F22.15.2990"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.270.22.13298"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.270.22.13298"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1208540"}],"container-title":["Lifestyle Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/165374","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/165374","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:48:52Z","timestamp":1745455732000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000165374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10,24]]},"references-count":10,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2009,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000165374","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.5120503","asserted-by":"subject"}]},"ISSN":["2504-3161","2504-3188"],"issn-type":[{"value":"2504-3161","type":"print"},{"value":"2504-3188","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10,24]]}}}