{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T21:53:37Z","timestamp":1777758817719,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2008,10,28]],"date-time":"2008-10-28T00:00:00Z","timestamp":1225152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2008,10,28]],"date-time":"2008-10-28T00:00:00Z","timestamp":1225152000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Nephrol"],"abstract":"<jats:p>To evaluate the importance of tubular proteinuria in diabetic nephropathy, we studied the serial changes of micro-albuminuria, microproteinuria and protein patterns by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) in 38 diabetic patients over 8 months. There was a significant correlation between the amount of micro-albuminuria measured by radio-immunoassay and the amount of microproteinuria quantitated by the Coomassie brilliant blue dye binding method (r = 0.976; p &lt; 0.0001). Micro-albuminuria of 50 mg\/day was equivalent to microproteinuria of 190 mg\/day. Among the 38 diabetic patients, 26 had micro-albuminuria above 50 mg\/day, while 12 had micro-albuminuria below this level. There was a significant correlation between the amount of microproteinuria and haemoglobin Ai, showing that the quantity of microproteinuria was affected by metabolic control. Diabetic patients with micro-albuminuria of above 50 mg\/day have a significantly higher diastolic blood pressure than those below this level. Among the diabetic patients with micro-albuminuria of less than 50 mg\/day, the amount of micro-albuminuria and microproteinuria remained constant, whereas progressive increases in microalbuminuria and microproteinuria were observed among the 12 diabetic subjects with micro-albuminuria above 50 mg\/day. These support the prognostic importance of this quantity of micro-albuminuria. The protein patterns as revealed by SDS-PAGE with Coomassie blue staining show a significant loss of low-molecular-weight proteins in 7 patients, which may therefore suggest tubular damage. The loss of tubular proteins persisted over a period of 8 months in all 7 subjects, and the amount gradually increased over this period. To conclude, our results indicate that diabetic proteinuria is characterized by urinary loss of albumin as well as low-molecular-weight proteins. The latter is associated with tubular damage, which may be one of the manifestations of the renal complications of diabetes mellitus.<\/jats:p>","DOI":"10.1159\/000168172","type":"journal-article","created":{"date-parts":[[2008,10,29]],"date-time":"2008-10-29T11:30:15Z","timestamp":1225279815000},"page":"475-481","source":"Crossref","is-referenced-by-count":11,"title":["Eight-Month Longitudinal Study of Urinary Excretion of Albumin and Tubular Proteins in Diabetic Subjects"],"prefix":"10.1159","author":[{"given":"Kin-kee","family":"Pun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Lau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis","family":"Hon-wai Wong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,10,28]]},"container-title":["American Journal of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000168172","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:14:29Z","timestamp":1777468469000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000168172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10,28]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000168172","archive":["Portico"],"relation":{},"ISSN":["0250-8095","1421-9670"],"issn-type":[{"value":"0250-8095","type":"print"},{"value":"1421-9670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10,28]]}}}