{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:51:54Z","timestamp":1745470314126,"version":"3.40.4"},"reference-count":0,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[1987,1,1]],"date-time":"1987-01-01T00:00:00Z","timestamp":536457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1987,1,1]],"date-time":"1987-01-01T00:00:00Z","timestamp":536457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Kidney Blood Press Res"],"published-print":{"date-parts":[[1987]]},"abstract":"<jats:p>This study systematically evaluates the effect of changes in the acid-base composition of the incubation media on the electrogenic H&lt;sup&gt;+&lt;\/sup&gt; and HCO&lt;sup&gt;-&lt;\/sup&gt;&lt;sub&gt;3&lt;\/sub&gt; secretion (voltage clamp method and serosal ouabain) in the isolated turtle urinary bladder. Since the various cell types would change their acidity in a similar direction but to a variable degree, measured mean cell pH values (5, 5-dimethyl-2,3-oxazolidinedione method) were used for an overall assessment of the changes in the acid-base status of the acid-transporting cells. Although addition of exogenous CO&lt;sub&gt;2&lt;\/sub&gt; (0.7\u20133%) increased H&lt;sup&gt;+&lt;\/sup&gt; secretion (JH&lt;sup&gt;+&lt;\/sup&gt;) 2- to 4-fold from a CCVfree control period, a further increase in the percent of CO&lt;sub&gt;2&lt;\/sub&gt; did not enhance JH&lt;sup&gt;+&lt;\/sup&gt; demonstrating a permissive but not a stimulatory role of CO&lt;sub&gt;2&lt;\/sub&gt;. Cyclic AMP plus 3-isobutyl-l-methylxanthine-induced electrogenic HCO&lt;sub&gt;3&lt;\/sub&gt; secretion (JHCO&lt;sup&gt;-&lt;\/sup&gt;&lt;sub&gt;3&lt;\/sub&gt;) remained unaltered at 10% CO&lt;sub&gt;2&lt;\/sub&gt; from a 5% CO&lt;sub&gt;2&lt;\/sub&gt; control period. Cell acidosis resulting from either alterations in the PCO&lt;sub&gt;2&lt;\/sub&gt;\/HCO&lt;sup&gt;-&lt;\/sup&gt;&lt;sub&gt;3&lt;\/sub&gt; levels or from NH4CI in the bathing solution did not enhance JH&lt;sup&gt;+&lt;\/sup&gt;; by contrast maximal levels of acidification were found at cell pH values of about 7.40 and comparable effects on JH+ were found with a variety of PCO&lt;sub&gt;2&lt;\/sub&gt;\/HCO&lt;sup&gt;-&lt;\/sup&gt;&lt;sub&gt;3&lt;\/sub&gt; combinations that led to a similar intracellular acidity.<\/jats:p>","DOI":"10.1159\/000173116","type":"journal-article","created":{"date-parts":[[2008,11,7]],"date-time":"2008-11-07T10:16:19Z","timestamp":1226052979000},"page":"69-84","source":"Crossref","is-referenced-by-count":0,"title":["Marginal Effect of Changes in Acid-Base Status in vitro on Rheogenic H&lt;sup&gt;+&lt;\/sup&gt; and HCO&lt;sup&gt;-&lt;\/sup&gt;&lt;sub&gt;3&lt;\/sub&gt; Secretion in Turtle Urinary Bladder"],"prefix":"10.1159","volume":"10","author":[{"given":"Horacio J.","family":"Adrogu\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry","family":"Tasby","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Ulate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,11,7]]},"container-title":["Kidney and Blood Pressure Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/173116","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/173116","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:30:14Z","timestamp":1745454614000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000173116"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2004,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000173116","archive":["Portico"],"relation":{},"ISSN":["1420-4096","1423-0143"],"issn-type":[{"type":"print","value":"1420-4096"},{"type":"electronic","value":"1423-0143"}],"subject":[],"published":{"date-parts":[[1987]]}}}